Journal of Proteomics & Bioinformatics
Open Access

Chitosan/Wollastonite composite scaffold preparation by freeze gelation method

U Sindhu, N Panda and K Pramanik

: JPB

Abstract :

Tissue engineering offers an alternative technique to tissue transplantation for diseased or malfunctioned organs and donor tissue shortages. It includes three basic components like scaffolds, cells and growth factors and signals to create three-dimensional tissues. Scaffolds should be porous, biodegradable, biocompatible, nontoxic and easily colonized by cells. Scaffold architecture and its properties greatly influence the success of tissue engineering. The main aim of this work is to design composite scaffold using chitosan and wollastonite by freeze gelation method and their characterization. Composite scaffolds are fabricated using chitosan, a biodegradable polymer and wollastonite, an inorganic material by freeze gelation method. Thermally induced phase separation principle is involved in this method. It results in the production of isotropic, highly interconnected, and porosity-designed membranes. Addtion of wollastonite enhances the bioactivity and mechanical strength of the composite scaffold. The porosity, crystallinity and pore size can be characterized using scanning electron microscopy (SEM), differential scanning calorimeter (DSC), Mercury porosimeter, and X-ray diffraction (XRD).