Chemopreventive effects of a curcumin-like diarylheptanoid [2,6-b | 9068
Rheumatology: Current Research

Rheumatology: Current Research
Open Access

ISSN: 2161-1149 (Printed)


Chemopreventive effects of a curcumin-like diarylheptanoid [2,6-bis(2,5-dimethoxybenzylidene) cyclohexanone] in cellular targets of rheumatoid arthritis in vitro

8th International Conference of Orthopedic Surgeons and Rheumatology

March 22-23, 2017 Rome, Italy

Syahida Ahmad

Universiti Putra Malaysia, Malaysia

Posters & Accepted Abstracts: Rheumatology

Abstract :

Statement of the Problem: Synovial fibroblast has emerged as a potential cellular target in progressive joint destruction in rheumatoid arthritis development. Our preliminary findings had shown that the newly synthesized curcumin analogue [2,6-bis(2,5- dimethoxybenzylidene) cyclohexanone] or BDMC33 exhibited improved anti-inflammatory activity by inhibiting nitric oxide synthesis, PGE2 synthesis and cyclooxygenase (COX) expression in activated macrophage cells. Aim: Aim of this study was to investigate the potency of BDMC33 on molecular and cellular basis of synovial fibroblasts (SF) in vitro. Methodology & Theoretical Orientation: Synovial fibroblast cells (HIG-82) were cultured in vitro and induced by phorbol-12- myristate acetate (PMA) to stimulate the expression of matrix metalloproteinase (MMPs) and pro-inflammatory cytokines. The protective effects of BDMC33 were evaluated toward MMP activities, pro-inflammatory cytokine expression and nuclear factor kappa-B (NF-KB) activation by using various bioassay methods, including zymography, western blotting, reverse transcription polymerase chain reaction, immunofluorescence microscopy and electrophoretic mobility shift assay. Results: The results showed that BDMC33 significantly inhibited the pro-gelatinase B (pro-MMP-9) and collagenase activities via suppression of MMP-1 in activated SF. In addition, BDMC33 strongly suppressed MMP-3 gene expression as well as inhibited COX-2 and IL-6 pro-inflammatory gene expression. We also demonstrated that BDMC33 abolished the p65 NF-KB nuclear translocation and NF-KB DNA binding activity in PMA-stimulated SF. Conclusion & Significance: BDMC33 represents an effective chemo-preventive agent and could be used as a promising lead compound for further development of rheumatoid arthritis therapeutic intervention.

Biography :