Current Synthetic and Systems Biology
Open Access
ISSN: 2332-0737
ISSN: 2332-0737
Jeevithan Elango and Wu Wen Hui
Shanghai Ocean University, China
Posters-Accepted Abstracts: Curr Synthetic Sys Biol
Type-II pepsin soluble collagens (PSC) were isolated from cartilage of shark and examined for their biochemical and antioxidant properties. The purifiedcollagen was composed of an identical (�?±1)3 chains. The biochemical properties such as viscosity, solubility, amino acid composition, glycoprotein content, SEM, FTIR, CD and DSC spectra and antioxidant properties substantiated that PSC could be used as a suitable biomaterial for pharmaceutical and biomedical industriesas an alternative source of mammalian collagen. From the gut-sac (in vitro intestinal absorption) experiments, weidentified hydrolysates within a MW range from 70 kD to 8 kD on the serosal side of the intestine after the application of collagen hydrolysates and a significant amount of collagen hydrolysate is absorbed in the molecular form of 35-40 kDa. In continuation, type-II collagen ouspolypeptide (37 kDa) was successfully separated fromthe hydrolysates and studied for theirphysico-functional and antioxidant properties. Further, we target to confirm the effects of oral tolerance of PSC on inflammatory and immune responses to the ankle joint of rheumatoid-arthritis (RA) ratsinduced by Complete Freundâ�?�?s adjuvant.The immunological indexes such as delayed type hypersensitivity, IL-10 and T cell apoptosis showed that PSC with concentration 10 �?¼g/L could have significant effect in the model. The result of histological staining indicated that the recovery of the articular membranes of ankle joint in PSC group was greatly enhanced. In conclusion, the present results suggest that appropriate dose of PSC can not only ameliorate symptoms but also modify the disease process of RA.
Email: srijeevithan@gmail.com