Journal of Hepatology and Gastrointestinal disorders
Open Access
ISSN: 2475-3181
ISSN: 2475-3181
Leonid Pavlovskyi
National Medical Universit, Ukraine
Posters & Accepted Abstracts: J Hepatol Gastroint Dis
Introduction: Sphingolipids are biological active components of all cell membrane. They play a great role in cell interconnections and take part in such process as a proliferation, maturation and cell apoptosis. Ceramide deserve special an attention, because they could be precursors of apoptosis and lead to development of Diabetes Mellitus type 2 (DM type 2) and Nonalcoholic Fatty Liver Disease (NAFLD). Objective: To investigate dependence between ceramide levels in plasma, level of insulin and HOMA indices in patients with DM type 2 and activity of AST, ALT in patients with NAFLD. Materials & Methods: We performed researches in three groups. The total number of patients was 60 (n=60). Two of them consisted of patients with DM type 2 and NAFLD (n=20, n=20, respectively) and the third one was healthy persons. The level of insulin, HOMA indices and activity of AST and ALT were assessed by using common biochemical blood analyses. Plasma ceramides (C14:0, C16:0, C18:0, C18:1, C20:0, C24:0 and C24:1) were quantified using electrospray ionization tandem mass spectrometry after separation with HPLC. Results: As result of researches, we got data that show high levels of ceramide in the first and second group comparing with healthy persons. This result is tightly correlates with the high level of insulin, HOMA indices and activity of AST and ALT in patients with DM type 2 and NAFLD, respectively. Conclusion: During our researches, we found out dependence between high ceramide levels with severity of DM type 2 and activity rate of NAFLD. So, further investigation of ceramide is needed for taking them as parameters for prognosis DM type 2 severity or as development NAFLD complications such as nonalcoholic steatohepatitis.
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