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Brawijaya University, Indonesia
Posters-Accepted Abstracts: Endocrinol Metab Syndr
Most of biological signaling pathway was orchestrated at the level of proteins and biological active compounds, that both molecules recognition have ability to interact and modulate molecular mechanisms underlying an organismâ�?�?s physiological functions. Differentiation of structural and physicochemical properties of bioactive compounds is an important factor to determine the biological function. Local Indonesian goat Ethawah breed milk is one of the nutritionally enriched dairy breed milk to provide biological function modulation without health risks. Recently in our study, we found high amount of caprine alpha-S2 casein of goat Ethawah breed milk exclusively isolated from 36 kDa, and identified the eight bioactive peptides fragments of caprine alpha-S2 casein by MALDI-TOF analysis. This study focuses on elucidating the biological function of caprine milk alpha-S2 casein protein provided by in vitro and in silico analyses. We found that alpha-S2 casein protein in high MG environment inhibits the decreasing of viability due to increase in proliferation of MC3T3E1pre-osteoblast cell. In animal model, this protein was able to down regulate cytokine proinflammation on some tissues target and controlled RAGE-AGE signaling pathway related with ROS and oxidative stress. These data was also confirmed by in silico analysis, and we detected that each bioactive peptide of caprine milk alpha-S2 casein protein displays a wide range of physiological functions as anti-inflammation, anti-oxidative, and immunomodulatory mechanism to regulate the cellular and molecular signaling mechanism to promote health life improvement.