Drug Designing: Open Access
Open Access

Can matrix effect in LC/MS or LC/MS/MS assay be avoided or fully compensated?

JOINT EVENT ON 3^rd International Conference and Expo on DRUG DISCOVERY & DESIGNING and 9^th Annual PHARMACEUTICAL CHEMICAL ANALYSIS CONGRESS

October 02-03, 2017 | Vienna, Austria

Miroslav Ryska

Quinta-Analytica, Czech Republic

Keynote: Drug Des

Abstract :

The source of the â�?�?Matrix effectâ�? as a consequence of analyte ions suppression or ions enhancement must be sought in the presence of unknown impurities from matrix. They are participating in the complex ionization process in parallel or competing ion-molecular reactions. Not only impurities from extracts but impurities adsorbed in the ion source and/or in the analytical system may play an important role. These adsorbed substances cannot be fully removed from the system by any cleaning procedure. To fully compensate for the negative impact of the â�?�?Matrix effectâ�?�?, use of isotopically labeled internal standards (isotope dilution technique) are proved to be the only effective technique. This applies especially to LC/MS/MS determination of drugs and their metabolites in complex extracts of biological matrices. The isotope dilution technique is successful regardless of the method of purification, the ionization technique (APCI or ESI) and the type of the equipment used. The isotope dilution technique proved to be 100% effective for the compensation of matrix effect influences in 132 analytical methods developed and validated. The strict requirements of EMA guidelines to investigate different plasma sources for the assessment of the matrix effect in the analytical method validation are discussed.

Biography :

Miroslav Ryska holds an Undergraduate Degree from Charles University, along with an MS in Physical Chemistry from Moscow State University and a PhD from the Institute of Macromolecular Chemistry of Czechoslovak Academy of Sciences. From 1961 to 1978, he worked at the Institute of Macromolecular Chemistry of the Czechoslovak Academy of Sciences. From 1978-1997, he worked as a Researcher in the Research Institute for Pharmacy and Biochemistry in Prague. He has written more than 100 publications mainly on the topic of mass spectrometry, trace analyses, analyses of drugs, metabolites and quantitative analysis. Currently he is in the position of Scientific Advisor.