Xi-Ying Luan, Guo-Yan Wang, Si-Ying Zhang and Fei-Fei Wang
Accepted Abstracts: J Cell Sci Ther
It is reported that human placenta derived mesenchymal stem cells(hPMSCs) have strong immunosuppressive properties and exhibit immune-related cell surface markers similar to bone marrow derived mesenchymal stem cells, including being positive for HLA-ABC but negative for HLA-DR and a number of costimulatory molecules, such as PDL1, a negative costimulatory molecule related to T cell activation. Here we investigated whether PDL1 expression on hPMSCs involved in the adhesion and migration of hPMSCs. Culture-expanded hPMSCs showed the typical appearance, immunophenotype, mutiple diff erentiation capacity, and highly expressed PDL1. RT-PCR and immunofl uorescence analysis showed that PDL1 siRNA could be successfully transfected into hPMSCs via liposome transfection method, and the expression of PDL1 could be effi ciently blocked. Cell count indicated that the diff erence of hPMSCs adhesion rate between blockade group and control group was no statistically signifi cant which was observed at half an hour aft er cell inoculation; but at one hour or three hours aft er inoculation, the adhesion rate of hPMSCs was signifi cantly higher in the blocked group than that in the control group(P<0.05). Transwell cell culture system assay showed that hPMSCs migration numbers in blockade group was not signifi cantly diff erent with that in the control group under the culture conditions of SDF1α. However, under the condition of DMEM complete medium or hPMSCs culture supernatant, the migration number of hPMSCs in blockade group signifi cantly reduced (P<0.05). Th us we conclude that PDL1 played an important role in the adhesion and migration of hPMSCs, which provides the theoretical basis for the further study of the biological signifi cance of the costimulatory molecules, such as PDL1, expression on hPMSCs.
Xi-Ying Luan, PhD, is the professor of immunology. She has completed her PhD from Soochow University. She is the director of Immunology, and assistant Dean of School of Basic Medical, Binzhou Medical university. She has published more than 35 papers in reputed journals. She is also a member of the editorial board, Journal of Clinical Rehabilitative Tissue Engineering Research. Dr Luan?s research focuses on the molecular mechanisms of costimulatory molecules involved in the regulatory roles of mesenchymal stem cells on immunocytes.