Afeesh Rajan Unnithan, Arathyram R S, Chan Hee Park and Cheol Sang Kim
Chonbuk National University, Republic of Korea
Posters-Accepted Abstracts: J Pharma Care Health Sys
Scaffolds play very crucial role in bone tissue engineering. Scaffolds are three dimensional bioactive structures with proper mechanical strength, cytocompatibility and should provide a better template for cell attachment to stimulate bone tissue formation. Hydroxyapatite (HaP) is the major constituent of natural bone with excellent biocompatibility and bioactivity and hence it has been widely explored for hard tissue engineering. In order to overcome the poor fracture toughness of hydroxyapatite, it has been combined with other bioactive materials. In this regard, Graphene Oxide (GO) is particularly attractive, since it provides mechanical integrity, large aspect ratio and it does not compromise the inherent bioactivity of hydroxyapatite. Moreover, the carboxyl functional groups bound to the surface of GO are expected to enhance the interfacial interaction between GO and the bioactive polymers such as Chitosan (CS) and Hyaluronic Acid (HA). Graphene oxide and chitosan are promising materials for tissue regeneration. HA is well known to be a major component of the natural extracellular matrix of nearly all mammalian connective tissues. The present study explores a novel GOâ�?�?CS-HA biomimetic composite scaffold containing an osteogenesis-inducing drug Simvastatin (SV). The composite scaffold can function as a template material for the biomineralization of HaP, which is crucial for bone regeneration. The bioinspired surface induced nucleation of HaP in a simulated physiological environment. The combination of GO-CS-HA conjugate induced biomineralization contributed in favorably towards the cytocompatibility such as osteoblast cell attachment and proliferation. Furthermore, the release of simvastatin has been known to improve the bone anabolic effects. Cytotoxicity and cell viability study suggest that SV loaded composite scaffold have proper biocompatibility to be used as a scaffold which can induce osteogenesis. So the composite biomimetic scaffold developed in the present study can be used for bone tissue regeneration.