Journal of Cell Science & Therapy
Open Access
ISSN: 2157-7013
ISSN: 2157-7013
Mohamed Mahmoud
Ain Shams University, Egypt
Scientific Tracks Abstracts: J Cell Sci Ther
The aim of this research was to test the magnetic power to attract or/and deviate the mesenchymal stem cells laden with nanoparticles of superparamagnetic iron oxide. Mesenchymal stem cells were obtained from Wharton��?s jelly of the umbilical cord and labeled with superparamagnetic iron oxide particles. The cell suspension was kept at 3 ml plastic tube as well as a 7 cm diameter petri��?s dish. Different intensities of static magnetic field were applied by introducing a magnet to one side of the tube and the dish for 5 minutes. Gentle shaking of the suspension was applied manually. Naked eye observation: A condensation of cells was noted at the side of the magnets that gave a light pinkish brown color with a rather clearer medium in both the tube and petri��?s dish. Moreover, with time there were aggregates of macroparticles of few mm sizes with a dark brown coloration. Furthermore, after removal of the magnets this phenomenon continues for more than 30 minutes denoting that the superparamagnetic nano-particles acquired magnetism. Microscopic examination: Fresh samples were taken from both sides of the tube and Petri��?s dish representing the nearest and the most fare points from the magnet and examined under the inverted light microscope. There were highly condensed cells with intact protoplasm of the near point in comparison with that of the fare point that showed a sparse cellular distribution with intact cytoplasm too. A series of research is required in order to test for the effect of static and kinetic magnetic power on these labeled cells as well as to test for vitality, liability, multiplicity and safety of these cells. We hope to be able to settle mesenchymal stem cells at certain tissue in vivo by of these cells by the use of this phenomenon.
Mohamed Mahmoud gained MBBCh in 1981, with a grad excellent with honor of medicine, Ain Shams University. He did his Master degree in 1985, internal medicine, with excellent grad of medicine at Ain Shams University and Doctor Degree in 1990, internal medicine of medicine, Ain Shams University. Then he was Resident at the endocrine unit, faculty of medicine, Ain Shams University for 3 years starting 1982. He was posted as Assistant lecturer, at the endocrine unit, faculty of medicine, Ain Shams University for 4 years starting 1985 and as Lecturer, at the endocrine unit, faculty of medicine, Ain Shams University for 5 years starting 1990. Then he got the post of Assistant professor, at the endocrine unit, faculty of medicine, Ain Shams University for 5 years starting 1995 and promoted to Professor, at the endocrine unit, faculty of medicine, Ain Shams University starting 2001 till now.
Email: mohamedsaad03@yahoo.com