Current Synthetic and Systems Biology
Open Access

Artificial RNA Synthesis Can Be Facilitated by a 5S rRNA Cassertte

International Conference on Synthetic Biology

September 28-29, 2015 Houston, USA

George E Fox

University of Houston, USA

Posters-Accepted Abstracts: Curr Synthetic Sys Biol

Abstract :

Small Artificial RNAs are being used in a variety of applications. Synthesis of such RNAs in large quantity by traditional means can be prohibitively expensive. An alternative approach has been devised in which the RNA of interest is embedded in a gene encoding a 5S ribosomal RNA variant that is very stable but is not incorporated into ribosomes. It is shown that a large variety of variants can be stabilized by inclusion in this cassette when expressed in Eschericia coli. The casette carrying the artificial RNA is expressed using the usual ribosomal RNA promoters and the product RNA accumulates in numbers comparable to those of wildtype 5S rRNA, which in the range of 50-100,000 copies per cell . The artificial RNA product degrades slowly allowing adequatetime for harvesting. Depending on the application, in some cases the RNA can be used without removal from the casette. If it is necessary to remove the artificial RNA this can be accomplished using specifically targeted DNAzymes, which can subsequently be recycled. Transcripsonal studies have shown that the only effect of the presence of the artificial RNA is an increase in the expression of genes involved in nucleotide degradation.

Biography :

Email: fox@uh.edu