Application of ultra performance convergence chromatography in fo | 20162
Journal of Nanomedicine & Biotherapeutic Discovery

Journal of Nanomedicine & Biotherapeutic Discovery
Open Access

ISSN: 2155-983X

+44 1300 500008

Application of ultra performance convergence chromatography in food safety

Joint Event on 30th Annual Congress on Nanotechnology and Nanomaterials & 8th World Congress on Spectroscopy and Analytical Techniques

September 10 - 11, 2018 I Stockholm, Sweden

Guo Wei

Chinese Academy of Inspection and Quarantine, China

Posters & Accepted Abstracts: J Nanomedine Biotherapeutic Discov

Abstract :

The development of greener analytical techniques is a topic of great interest. There is an increasing need for new analytical methods that can handle a large number of analytes in food and biological samples. Ultra performance convergence chromatography (UPC2) is considered a valuable tool helping to separate and determine compounds differing by subtle structural differences. UPC2 presents several advantages over high performance liquid chromatography (HPLC); it takes less column equilibration time and consumes less organic reagents. UPC2 has recently been successfully used to separate and determine a lot of analytes, including many pharmaceutical compounds. In this work, recent applications of UPC2 for the analysis of different compounds in food and biological samples were reviewed, in the hope of helping chromatography users to have a new look on the possibilities offered by this technique. Furthermore, a simple, highly sensitive and fast analytical method based on UPC2 with photo-diode array detection (PDA) has been developed to quantify 15 sulfonamides and their N4-acetylation metabolites in serum. Variables affecting the UPC2 were optimized to get a better separation. We provide a discussion of stationary phase, modifiers and additives, column temperature, flow rate, and automatic back pressure regulator (ABPR) backpressure for UPC2 analysis and detection sensitivity. The performance of the developed method was also evaluated.

Biography :

Guo Wei has completed her PhD from China Agricultural University (CAU). She is the Vice Director of Institute of Food Safety in Chinese Academy of Inspection and Quarantine. Her fields of research are application of chromatography/mass spectrum in food safety, medicinal analysis, proteomics, metabonomics, etc. She has finished over 20 research projects successfully, published more than 15 research papers in international top-tier academic journals, and two books written in Chinese and English.