Apoptosis in the human laryngeal carcinoma (Hep-2) cell line by Bulbine natalensis and B. frutescens fractions
Biochemistry & Pharmacology: Open Access

Biochemistry & Pharmacology: Open Access
Open Access

ISSN: 2167-0501

Apoptosis in the human laryngeal carcinoma (Hep-2) cell line by Bulbine natalensis and B. frutescens fractions

International Conference on Pharmacognosy, Phytochemistry & Natural Products

October 21-23, 2013 Radisson Blu Plaza Hotel, Hyderabad, India

Lalini Reddy and Singh Rishan

Scientific Tracks Abstracts: Biochem & Pharmacol

Abstract :

Many plants that belong to the genus Bulbine have compounds in their roots and leaves which are considered important for traditional treatments. The stems and roots of Bulbine species are believed to contain anticancer compounds such as anthraquinones, including chrysophanol and knipholone. However, in general, people utilise plants of the Bulbine genus for the treatment of rashes, itches, wounds, burns, cracked lips and cracked skin. This study assessed the effect of aqueous and organic fractions of Bulbine natalensis and Bulbine frutescens on the human laryngeal carcinoma cell line (HEp-2) for apoptosis. The MTT assay was used to determine the cytotoxicity of the fractions administered and to select fractions for analysis of bax and caspase-3 gene expression, which are induced during programmed cell death type 1, known as apoptosis. All of the B. natalensis fractions induced expression of caspase-3, while the tested B. frutescens aqueous root fractions failed to induce expression of caspase-3. The variation in bax gene expression indicated that HEp-2 cell death was due to apoptosis and other unknown forms of cell death that may or may not activate caspase-3 gene expression.

Biography :

Lalini Reddy has completed her doctorate degree in Biotechnology at the age of 41 years from Durban University of Technology. She has been a faculty member teaching undergraduate and supervising postgraduate students for the last 26 years. She has published in highly reputable peer-reviewed journals and has served as a reviewer for several journals. She has surveyed more than 30 traditional South African plants for their medicinal potential.