Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
+44 1478 350008
ISSN: 2161-0495
+44 1478 350008
Kermandji Mohamed Azed, Boubellouta Houria, Khelifi Touhami Fatima, Abidli Nacira and Bellatrache Cherifa
University of Bejaia. Bejaia. Algeria.
Department of Animal Biology, University of Constantine, Algeria.
Department of Veterinary Medicine, University of Constantine, Algeria.
USTHB, Algeria.
Department of Clinical Biochemistry, University of Constantine, Algeria
Posters & Accepted Abstracts: J Clin Toxicol
The present survey is centered on protective effect of Matricaria pubescens against liver injury induced by carbon tetrachloride in rats. In our study, we indicated that these plants treatment have a potent protective effect as revealed by remarkable decrease in MDA content, additionally, methanolic extract could ameliorate acute liver damage to a high degree, as demonstrated by reduction of serum AlT levels. Introduction: The liver is a target organ for the many chemical products. CCL4 is a classically known compound that causes hepatotoxicity by an acute exposer (Recknagel, 1967 ). Matricaria pubescens plant of family compositea used in algeria for the treatment of hepatic diseases. The main objective of the present work was to evaluate the hepatoprotective and antioxidant activity of Matricaria pubescens against liver injury induced by carbon tetrachloride in rats. Materials and methods: 1. Extraction of methanolic extract: The aerial parts (soft twigs and leaves) of Matricaria pubescens is air-dried and extracted with methanol. 2. Determination of free radical scavenging activity: The free radical scavenging activity of the extract was determined by the method described by (Burits and Bucar, 2000). 3. Determination of total phenolic content and flavonoids: Total soluble phenolics in the methanol extract of Matricaria pubescens were determined with Prussian bleau according to the method of Price and Butler (1977), with slight modifications of (Graham 1992), using gallic acid as a standard compound. 4. The flavonoids content in the methanol extract of Matricaria pubescens was determined by ( Boharun et al., 1996) method using quercetin and rutin as a reference compound. 4. Pharmacological essays (Study carry out on rats ) Conclusion: Our investigation provided convincing data that Matricaria pubescens have an impressive hepatoprotective effects on acute liver injuries induced by CCL4. The mechanisms underlying hepatoprotection of the methanolic extract of Matricaria pubescens may be related to both its radical scavenging properties and indicate effects as a regulator of antioxidative systems, which might be considered to be therapeutic in clinical situations.