Journal of Pharmaceutical Care & Health Systems
Open Access
ISSN: 2376-0419
+44 1300 500008
ISSN: 2376-0419
+44 1300 500008
Sarne Paul Jazon I, Verzosa Dhennis T, Dayrit Jhulez Anthony B, Gonzales Thea Frances Ruth N, Yu Raymund B, Larcia Levi-Letlet H and Matias Ronald R
University of the Philippines Manila, Philippines
United Laboratories Inc., Philippines
Scientific Tracks Abstracts: J Pharma Care Health Sys
Alkaloidal extracts from Nephelium lappaceum L. (Rambutan) were investigated for selective anti-Staphylococcal and anti-MRSA activities. Ethanolic extracts of the pericarp, seeds and leaves were extracted for alkaloids (through a modified Stas-Otto Method I) and had undergone bioassay-guided fractionation via C18 SPE. Resazurin assays were done to determine MIC on clinically isolated Staphylococcus aureus and an MRSA variant. It was also used to determine safety on MDCK cell line (a non-cancerous mammalian cell line). Selectivity Index (SI), a ratio of the MDCK IC50 to MIC, was used as the measure of safety. The Ethanolic Pericarp Extract (EPE) was potently inhibitory to both S. aureus and MRSA, but was not selective (Sa & MRSA MIC=500 �?¼g/mL, SI= 0.86). The alkaloid extract had similar MIC against MRSA but was more selective (Palk-B, SI>2.00), while the third fraction of the alkaloidal extract had greatly improved antibacterial effect and selectivity (PB-f3, Sa & MRSA MIC=125 �?¼g/mL, SI=5.22). Results suggest the presence of a potent and selective anti-Staphylococcal agent in PB-f3, which is also effective against MRSA.
Sarne Paul Jazon I graduated with BS in Industrial Pharmacy from the University of the Philippines, Manila. He is currently working in United Laboratories Inc. as a researcher of Natural Products. This study on Alkaloids and Peptides is in cooperation with his group mates as a requirement under the program MS Pharmacy - Pharmaceutical Chemistry.
Email: pauljazonsarne@gmail.com