Endocrinology & Metabolic Syndrome
Open Access

Anti-diabetic and hypo-lipidemic activity of green synthesized silver nanoparticles and G. sylvestre extract on streptozotocin induced diabetic rats: An in vivo approach

Global Meeting on Diabetes and Endocrinology

July 23-24, 2018 Kuala Lumpur, Malaysia

Kalakotla Shanker and G Krishna Mohan

Jawaharlal Nehru Technological University Hyderabad, India

Posters & Accepted Abstracts: Endocrinol Metab Syndr

Abstract :

In recent times, nanomaterials being used in anti-diabetic studies for their exclusive properties such as small size, more surface area, biocompatibility and enhanced solubility. In view of this, present study aimed to evaluate the anti-hyperglycemic potential of Green Synthesized Silver Nanoparticles (GSSNPs) and Gymnema sylvestre extract. The crystalline nature of the BSSNPs was confirmed by X-ray diffraction; the characteristic peaks observed at 2θ=38.23º, 44.33º, 64.56º and 77.45º were corresponded to (111), (200), (220) and (311). The Scanning Electron Microscopy (SEM) and High Resolution Transmission Electron Microscopy (HRTEM) analysis divulges that the GSSNPs were spherical in shape. Energy Dispersive X-ray Analysis (EDAX) spectrum exhibit peaks for the presence of silver, carbon and oxygen atoms in the range of 1.0-3.1 keV. FT-IR reveals the binding properties of active bio-constituents responsible for capping and stabilizing GSSNPs. After successful synthesis and characterization, the GSSNPs were tested for toxicity using Acute Oral Toxicity analysis (AOT) by OECD-423 guidelines. Diabetes was induced by injecting streptozotocin (STZ) intraperitoneally (40 mg/kg, i.p.). The results showed increased blood glucose, cholesterol, triglycerides, LDL, VLDL, huge loss in body weight, downturn in plasma insulin. The Gymnema sylvestre extract (200 mg/kg, 400 mg/kg), GSSNPs (100 mg/kg, 200 mg/kg) and Metformin 250 mg/kg were administered to the diabetic rats. GSSNPs at dose level of 200 mg/kg (b.wt p.o.) showed significant inhibition of (p<0.001) blood glucose levels and lipid profile as compared with Gymnema sylvestre extract treated group. These detections revealed that GSSNPs possess potent anti-hyperglycemic and anti-hyperlipidemic activity and thus preferable over crude extract.

Biography :

E-mail: shankerkalakotla@gmail.com