Clinical & Experimental Cardiology
Open Access
ISSN: 2155-9880
+44 1300 500008
ISSN: 2155-9880
+44 1300 500008
Shishir Upadhyay, Manisha Rani and Monisha Dhiman
Central University of Punjab, India
Posters & Accepted Abstracts: J Clin Exp Cardiolog
Doxorubicin (DOX) often prescribed for anticancer medication once, confronted a restricted clinical use for its dose limited cardiotoxic nature by stimulating oxidative stress in the tissues. The reduced antioxidative defence system and elevated levels of intracellular ROS conclude in terms of cell injury through one or the other mechanisms. Medicinal plants are a rich natural source of secondary metabolites (flavonoids) that have known antioxidant characteristics. The objective of the study was to test the extracts of certain medicinal herbs for their cardio protective properties against doxorubicin-arbitrated toxicity. At first, antioxidant capacities of these extracts were determined by DPPH and Ferric reducing/antioxidant power assay. In vitro MTT assay followed by an assessment of the cytoprotective activity of the crude extracts on DOX-treated mammalian cell line were evaluated. Levels of intracellular ROS were determined by using a peroxide sensitive fluorescent probe, 2,7 dichlorofluoroscien diacetate. Lactate dehydrogenase (LDH) and creatine kinase (CK) levels were used as a means of cardiac tissue damage. The levels of cytochrome c, caspase 9 and caspase 3, were measured by Western blotting. It was observed that the aqueous extracts were modulating the toxic effects of DOX on cardiotoxicity.
Email: shishirr.upadhyay@gmail.com