Taghrid Gaafar, Rabab El Hawary and Omneya Osman
Accepted Abstracts: J Cell Sci Ther
I solation and comparison of cellular and molecular characteristics of MSCs derived from full term amniotic membrane (AM) & endometrium. Investigation of a possible therapeutic potential for AM MSCs in the female reproductive tract. Study Design & methods: Human amniotic membranes (AM) from term placentas (N=8), endometrial /stromal (by scrapping endometrium) from premenopausal hysterectomies (N=6) after mincing and enzymatic digestion were included in the study. Immunophenotyping by flow cytometry, Karyotyping, differentiation to mesoderm, RT-PCR analysis for transcription factors, WNT signaling pathways, pluripotency markers and HOXA genes were performed. Results: Generated cells showed morphologically and phenotypically the characteristics of MSCs from other sources with fairly comparable results. The cumulative population doubling was significantly higher in endometrium MSCs 25�5.2 compared to 17.2�4.5 for AM MSCs (P<0.001).They differentiated into osteogenic, adipogenic and chondrogenic lineages till passage 9. They possessed normal karyotype by passage 6. They expressed HOXA genes and differentialy pleuripotency markers. Intrauterine injection of GFP transfected AM MSCs into female Wistar rats (N=10), demonstrated the presence of AM cells with GFP expression after 14 days within several mice uterine cryosections. AM MSCs highly expressed genes involved in patterning of the Female reproductive tract. Conclusions: AM and endometrium derived MSCs share fairly similar morphological, cellular and molecular characteristics. They possess mesenchymal stemness potentials. Endometrial MSCs possess the highest expansion capacity. AM MSCs may have a potential for stem cell therapy in the female reproductive tract.