+44 1478 350008
Khaled M A Hassanein and Yasmin O El-Amir
Assiut University, Egypt
Jazan University, KSA
Posters & Accepted Abstracts: J Clin Toxicol
The ameliorating effect of thymoquinone (TQ), the major active ingredient of Nigella sativa seeds on titanium dioxide naonparticles (TiO2 NPs) induced toxicity and oxidative stress in Sprague-Dawley (SD) rats was investigated. 40 rats were divided into four equal groups. The first, second, third and fourth groups received TiO2 NPs, TiO2 NPs and TQ, TQ only for six weeks. The fourth group served as the control. Exposure to TiO2 NPs resulted in increased liver enzyme markers, oxidative stress indices, tumor necrosis factor alpha (TNF-�?±) and DNA damage by comet assay. TiO2 NPs resulted in decreased level of testosterone hormone. Histopathological alterations were also observed in the liver, brain, lung and testes. Transmission electron microscopy revealed changes in the hepatocytes cytoplasm related to the oxidative stress and presence of nanoparticles in the testicular tissues. Co-administration of TQ with TiO2 NPs decreased the level of liver enzymes, oxidative stress, TNF-�?± and DNA damage and ameliorate the level of testosterone. Furthermore, TQ increased the total antioxidant and glutathione (GSH) levels. In conclusion, TiO2 NPs induce hazardous effects in different organs and are closely related to oxidative stress. TQ have antioxidative and anti-inflammatory effect against the detrimental effect of TiO2 NPs.