Alterations of hematopoietic stem cells and associated marrow mic | 21199
Journal of Cell Science & Therapy

Journal of Cell Science & Therapy
Open Access

ISSN: 2157-7013

+44 1300 500008

Alterations of hematopoietic stem cells and associated marrow microenvironment in experimentally induced leukemia and Aplastic anaemia: An inverse correlation

International Conference & Exhibition on Cell Science & Stem Cell Research

29 Nov - 1 Dec 2011 Philadelphia Airport Marriott, USA

Sujata Law

Scientific Tracks Abstracts: J Cell Sci Ther

Abstract :

Bone marrow (BM) is the ultimate machinery of hematopoiesis in the adult vertebrate system physiology and is the residential lap of hematopoietic stem and stromal cell. Th e cywtokinetics of bone marrow Hematopoietic Stem Cells (HSC) are controlled by various regulatory factors like cytokines, growth factors, nourishing facto rs and many other signaling molecules to help cell survival, diff erentiation, proliferation, and apoptosis in health which, suggestively, suff ers deregulation in diseased states. HSC maintains hematopoiesis and homeostasis by contracting and expanding the pool of hematopoietic cells in the bone marrow where they can be identifi ed phenotypically as Sca1+ and c-kit+ cells. Failure to maintain such functional effi cacy by HSC in the Bone Marrow may produce Aplastic Anemia. On the contrary, a deregulated diff erentiation and hyper-regulated proliferation of HSC may be held responsible for leukemia induction in individuals. Indeed, HSCs and haematopoietic progenitor cells (HPCs) are vulnerable targets for such disease oriented dysregulation which bears close correlation with the bone marrow microenvironmental damage. In our experimental set up, we induced aplastic anemia by injecting busulfan and cyclophosphamide and leukemia by N-N? ethylnitrosourea intraperitoneally in inbred swiss albino mice. Th e present study aims at evaluating the possible mechanism(s) of deregulation in the bone marrow physiology with special reference to marrow stem and stromal cell surface receptor expression, cellular granularity, cell cycle status and overall marrow architecture. We also presented the results of studying long term marrow culture, marrow adherent and hematopoietic progenitor cell colony formation, STAT and other signaling proteins expression and ap optosis mechanism involved in aplastic anemia and leukemia. Th e investigations revealed an interesting correlation between disease initiation, progression and specifi c marrow microenvironmental components association with HSC in the BM microenvironment with particular reference to leukemia and aplastic anemia which present an inverse scenario between each other

Biography :

Dr. Sujata Law did her Ph.D on Stem Cell Biology from the University of Calcutta and postdoctoral studies in the fi eld of Signal Transduction from Bose Institute, Kolkata. She is Assistant Professor (Stem Cell Biology)in the Department of Biochemistry & Medical Biotechnology at Calcutta School of Tropical Medicine, Kolkata, India. She has published more than 28 Original Articles and Reviews in reputed journals.