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Alpha-lipoic acid produces bone-protective effects via modulating Nox4/ROS/NF-and#954;B and Wnt/ Lrp5/and#946;-catenin signaling pathways in H2O2-treated MC3T3-E1 cells and ovariectomy rats
Journal of Cell Science & Therapy

Journal of Cell Science & Therapy
Open Access

ISSN: 2157-7013

Alpha-lipoic acid produces bone-protective effects via modulating Nox4/ROS/NF-κB and Wnt/ Lrp5/β-catenin signaling pathways in H2O2-treated MC3T3-E1 cells and ovariectomy rats


5th World Congress on Cell & Stem Cell Research

March 23-25, 2015 DoubleTree by Hilton Chicago - North Shore, USA

Hui-Jun Sun, Chao Fu, Chang-Yuan Wang, Yue Jin, Qi Liu, Qiang Meng and Ke-Xin Liu

Posters: J Cell Sci Ther

Abstract :

Recently, increasing studies proved the relationship between oxidative stress and osteoporosis. And some studies have been done to detect the possible beneficial effects of Alpha-lipoic acid (ALA), a potent antioxidant, on osteoporosis in vivo and in vitro. However, the detailed mechanism(s) underlying the bone-protective action of ALA are still poorly understood. The present study aims to examine the mechanisms by which ALA produces bone-protective effects in vitro and in vivo on base of its antioxidant effects, thus the effects of ALA on H2O2-treated MC3T3-E1 pre-osteoblasts and ovariectomized osteoporosis rat model were investigated by using bone biomechanical testing, micro computed tomography, western blotting and qRTPCR analysis. The results showed that ALA promoted osteoblastic formation, inhibited osteoblastic apoptosis, increased OPG/RANKL ratio and enhanced bone formation in vitro and prevented bone loss in vivo. And the effects of ALA were via modulating Nox4/ROS/NF-κB and Wnt/Lrp5/β-catenin signaling pathways. The current study indicated that ALA might be beneficial for the prevention and therapy of osteoporosis clinically at the end of advanced studies.

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