Journal of Proteomics & Bioinformatics
Open Access

A novel platform for expressing multi-subunit protein complexes and pathways

International Conference on Protein Engineering

October 26-28, 2015 Chicago, USA

David Mead

Lucigen Corporation, USA

Keynote: J Proteomics Bioinform

Abstract :

Circular plasmids frequently are difficult to use for expression of multiple or large genes, repetitive or toxic proteins, and pathways or multi-component gene circuits. The inherent supercoiling of circular plasmids imparts instability for these and other complex gene sequences. The pJAZZ plasmid is a unique linear cloning vector that tolerates nearly any DNA sequence including AT-rich genes and highly repetitive sequences that are impossible to capture in typical circular vectors. pJAZZ has a cloning capacity of up to 40 kb and lacks the cloning bias inherent to circular plasmids, enabling the assembly of complex multi-gene systems. We are developing pJAZZ expression vectors for a variety of applications including expression of mega Dalton proteins, expression of multiple proteins (up to six currently) and production of metabolites from prokaryotic pathways. The vector includes improved light-inducible expression cassettes that have distinct advantages over small-molecule induction such as: Precise spatial control of expression; instantaneous initiation and termination of inducing agent; tunability and lack of toxicity or cross-reactivity. The light inducible gene circuit has proven to be significantly more efficient in the linear pJAZZ backbone than in circular vectors.

Biography :

David Mead leads Research and Development efforts for the Company’s research use only products. He has earned his PhD in Physiology and Biophysics at the University of Illinois–Champaign/Urbana. He is the Inventor of TA cloning and he is the co-author of fifty two publications.

Email: dmead@lucigen.com