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A new theranostic system based on endoglin aptamer conjugated flu | 18747
Journal of Biomedical Engineering and Medical Devices

Journal of Biomedical Engineering and Medical Devices
Open Access

ISSN: 2475-7586

+44 1223 790975

A new theranostic system based on endoglin aptamer conjugated fluorescent silica nanoparticles


International Conference on Pharmaceutical and Biomedical Engineering

October 16-17, 2017 Osaka, Japan

Yongxiang Zhao, Nuo Yang, Zixi Hu, Zhenghua Zhang, Jie Tan, Rong Zheng, Zongqiang Lai, Yanmei Li, Chaofan Zhou, Wenlin Gong, Guoqing Zhang, Duo Zheng, Jian He, Jianhong Zhong, Xinglu Jiang, Yong Huang, Sufang Zhou and Juntao Tan

Guangxi Medical University, China

Scientific Tracks Abstracts: J Biomed Eng Med Devic

Abstract :

Background & Aim: Tumor vessels can potentially serve as diagnostic, prognostic and therapeutic targets for solid tumors. Fluorescent dyes are commonly used as biological indicators, while photobleaching seriously hinders their application. In this study, we aim to generate a Fluorescent Silica Nanoparticles (FSiNPs) theranostic system marked by the mouse endoglin (mEND) aptamer, YQ26. Methods: A highly specific YQ26 was selected by using gene-modified cell line-based SELEX technique. FSiNPs were prepared via the reverse microemulsion method. The YQ26-FSiNPs theranostic system was developed by combining YQ26 with the FSiNPs for in vivo tumor imaging, treatment and monitoring. Results: Both in vitro experiments (i.e., cellular and tumor tissue targeting assays) and in vivo animal studies (i.e., in vivo imaging and antitumor efficacy of YQ26-FSiNPs) clearly demonstrated that YQ26-FSiNPs could achieve prominently high targeting efficiency and therapeutic effects via aptamer YQ26-mediated binding to Endoglin (END) molecule. Conclusion: This simple, sensitive and specific YQ26-FSiNPs theranostic system has a great potential for clinical tumor targeting imaging and treatment. References 1.Fonsatti E, Nicolay H J, Altomonte M, Covre A, Maio M (2010) Targeting cancer vasculature via endoglin/CD105: a novel antibody-based diagnostic and therapeutic strategy in solid tumors. Cardiovasc Res.; 86(1): 12-9. 2.Dallas N A, Samuel S, Xia L, Fan F, Gray M J, Lim SJ, et al. (2008) Endoglin (CD105): A marker of tumor vasculature and potential target for therapy. Clin Cancer Res.; 14(7): 1931-7. 3.Schmidt K S, Borkowski S, Kurreck J, Stephens A W, Bald R, Hecht M, et al. (2004) Application of locked nucleic acids to improve aptamer in vivo stability and targeting function. Nucleic Acids Res.; 32(19): 5757-65. 4.Sefah K, Shangguan D, Xiong X, O'Donoghue MB, Tan W (2010) Development of DNA aptamers using Cell-SELEX. Nat Protoc; 5(6): 1169-85. 5.Chen F, Hong H, Zhang Y, Valdovinos HF, Shi S, Kwon GS, et al. (2013) In vivo tumor targeting and image-guided drug delivery with antibody-conjugated, radiolabeled mesoporous silica nanoparticles. Acs Nano; 7(10): 9027-39.

Biography :

Yongxiang Zhao is the Director of the National Center for International Research of Biological Targeting Diagnosis and Therapy. His researches focus on biological targeting diagnosis and therapy for tumor, including the key targets and mechanism of malignant tumors occurrence and progress, homologous antitumor vaccines, research and development of heterologous oncolytic biological drugs and research and development of tumor bio targeted diagnostic reagents. He has 79 SCI papers published in international journals. He also has one International Academic award, one Ministerial and Provincial first prize, 30 national patents and 2 authorized international PCT patents.

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