Journal of Biomedical Engineering and Medical Devices
Open Access
ISSN: 2475-7586
ISSN: 2475-7586
Serap Saglik Aslan and Sarioglu Cem
Istanbul University, Turkey
Posters & Accepted Abstracts: J Biomed Eng Med Devic
In this study, a new isocratic high performance liquid chromatographic method was developed and validated for simultaneous analysis of Sildenafil and Dapoxetine in tablet dosage form. Chromatographic separation was achieved with a mixture of Acetonitrile:Phosphate buffer pH 9.5 in the ratios 70:30 (v/v) as the mobile phase and a C18 column. The detection wavelength was adjusted to 228 nm. Retention times were found as 2.7 minutes for Sildenafil and 6.1 minutes for Dapoxetine. The linear concentration ranges were 0.25-0.75 mg/ml for Sildenafil and 0.15-0.45 mg/ml for Dapoxetine. The Limit of Detection (LOD) and the Limit of Quantitation (LOQ) values were 0.318 �?¼g/ml and 1.060 �?¼g/ml for Sildenafil, 0.316 �?¼g/ml and 1.053 �?¼g/ ml for Dapoxetine, respectively. Recovery percentages were found to be 99.07-100.37% for Sildenafil and 99.43-100.11% for Dapoxetine. The method was applied to simultaneous analysis of Sildenafil and Dapoxetine in tablet dosage form. The proposed method is simple, rapid, selective and reproducible. It can be recommended to be used for simultaneous analysis of Sildenafil and Dapoxetine in pharmaceutical industry.
Serap Saglik Aslan has been working as a Professor in the Department of Analytical Chemistry, Faculty of Pharmacy, Istanbul University since 2010.