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Mass Spectrometry & Purification Techniques

Mass Spectrometry & Purification Techniques
Open Access

ISSN: 2469-9861


NMR spectroscopy is one of the principal techniques used to obtain physical, chemical, electronic and structural information about molecules due to the chemical shift of the resonance frequencies of the nuclear spins in the sample. Peak splittings due to J-or dipolar couplings between nuclei are also useful. NMR spectroscopy can provide detailed and quantitative information on the functional groups, topology, dynamics and three-dimensional structure of molecules in solution and the solid state.When placed in a magnetic field, NMR active nuclei (such as H or C) absorb electromagnetic radiation at a frequency characteristic of the isotope. The resonant frequency, energy of the radiation absorbed, and the intensity of the signal are proportional to the strength of the magnetic field. For example, in a 21 Tesla magnetic field, hydrogen atoms (commonly referred to as protons) resonate at 900 MHz.

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