Journal of Clinical and Cellular Immunology

Journal of Clinical and Cellular Immunology
Open Access

ISSN: 2155-9899

+44 1223 790975

Review Article - (2012) Volume 0, Issue 0

The Role of NFκB in T-lymphocyte Development and Function

Elisha De Valle1,2, Laurensius K. Lie1,2, Stuart P. Berzins3 and Raffi Gugasyan1,2,4*
1Centre for Immunology, Burnet Institute, Melbourne, Victoria, Australia
2Department of Immunology, Central Clinical School, Melbourne, Victoria, Australia
3Ballarat Cancer Research Centre, University of Ballarat, Victoria, Australia
4Department of Microbiology and Immunology, University of Melbourne, Melbourne, Victoria, Australia
*Corresponding Author: Raffi Gugasyan, Center for Immunolgy Burnet Institute, Melbourne, Victoria, Australia, Tel: 61-03-9282 2111, Fax: 61-03-9282 2100 Email:

Abstract

Initially identified as a nuclear factor in B cells, the family of NFκB transcription factors have since been found to operate in almost all cell types, regulating the transcription of a wide range of target genes. The NFκB signalling pathway is of particular importance to T lymphocytes, playing a prominent role in both T cell development and function. This review will focus on the current understanding of the roles of NFκB during thymic T cell development, with an emphasis on some of the emerging roles for NFκB signalling in regulating the development of non-conventional thymocyte lineages. We will also evaluate the function of NFκB signalling in the polarization of T-helper subsets in the periphery, and how NFκB intersects with other T cell-intrinsic pathways through mechanisms of signalling crosstalk. Dysregulated NFκB signalling is implicated in numerous disease states, and a thorough understanding of NFκB function during different phases of T cell development and function will be vital for optimal targeting in a therapeutic setting.

Abbreviations

NFκB: Nuclear Factor kappa B; RHD: Rel Homology Domain; TAD: Transactivation Domain; IκB: Inhibitor of NFκB; NLS: Nuclear Localization Signal; IKK: IκB Kinase; NIK: NFκB Inducing Kinase; DN: Double Negative; DP: Double Positive; SP: Single Positive; TCR: T cell Receptor; Treg: T Regulatory; NKT: Natural Killer T cell; Eomes: Eomesodermin; CBP: CREB-binding Protein; Id3: Inhibitor of DNA Binding 3; KLF2: Krueppel-like Factor 2; MHC: Major Histocompatibility Complex; BAFF: B-cell Activating Factor; RANKL: Receptor Activator of Nuclear Factor kappa-B Ligand; Itk: IL2-Inducible T-cell Kinase; Rlk: Receptor-like Kinase; TEC: Thymic Epithelial Cell; PLZF: Promyelocytic Leukaemia Zinc Finger protein; EBV: Epstein Barr Virus; APC: Antigen Presenting Cell; DC: Dendritic Cell; TFH: T-Follicular Helper; EAE: Experimental Autoimmune Encephalomyelitis; GC: Germinal Center; ALL: Acute Lymphocytic Leukemia; NHL: Non-Hodgkin Lymphoma; T-ALL: T cell acute lymphoblastic leukemias; Tpl2: Tumor Progression Locus 2; MAPK: Mitogen-Activated Protein Kinase; MAP3K: Mitogen-Activated Protein Kinase Kinase Kinase; ERK: Extracellular signal-regulated kinase; MoMuLV: Moloney Murine Leukemia Virus

Introduction

Essential to the survival of all living organisms is the ability to carefully regulate the process of gene transcription. A testament to this is the evolutionary conservation of the Nuclear Factor kappa B (NFκB) family of transcription factors [1,2]. Originally identified as a Nuclear Factor in B cells [1], the NFκB family operates in most cell types to regulate the transcription of a wide range of target genes, some of which control the critical processes of cell survival, differentiation and proliferation [3]. The NFκB signalling pathway is also of considerable importance to T lymphocytes, playing prominent roles in the developmental and functional differentiation of different T cell subsets. Most recently, roles for NFκB have been associated with non-conventional T cell lineages, such as T regulatory cells and innate T cells. In addition to these physiological roles, dysregulated NFκB signalling has been implicated in numerous disease states including inflammatory and autoimmune disorders, as well as many cancers [3]. The components of the NFκB pathway therefore represent an attractive therapeutic target, with great therapeutic potential.

The Family of NFκB Transcription Factors

The mammalian NFκB family of transcription factors consists of five members, (RELA (or p65), c-Rel, Rel-b, NFκB1 (p105/p50) and NFκB2 (p100/p52)), which share a conserved Rel Homology Domain (RHD) at the N-termini. The RHD is vital for NFκB dimerization, DNA binding, nuclear localization, and inhibitor binding [4]. On the basis of their C-terminal domains, NFκB proteins can be classified into two distinct subclasses. The NFκB1 and NFκB2 subunits exist as the precursor proteins p105 and p100, respectively. These proteins contain a high number of inhibitory ankyrin repeat sequences at the C-terminal end. Proteolytic cleavage removes the C-terminal region [5] to produce the DNA-binding proteins; p105 is cleaved to form p50, and p100 to p52. In contrast, the RELA, c-Rel and Rel-b subunits each possess C-terminal transactivation domains (TAD) and their transcriptional activity is not dependent on proteolytic cleavage. The NFκB transcription factors function as dimer proteins, forming either homo or heterodimer complexes. The NFκB dimer combinations each bind distinct 10bp DNA sequences (κB sites) and regulate the transcription of distinct yet overlapping sets of target genes [6]. The p50 and p52 subunits lack TADs, and are transcriptionally active only when dimerized with RELA, c-Rel or Rel-b [7]. Homodimers of p50 and p52 can therefore play an inhibitory role, suppressing the transcription of certain target genes by blocking access to κB sites.

Pre-existing dimers of NFκB transcription factors are found in the cytoplasm of naïve T cells. This allows for rapid transduction of signals through the NFκB pathway, which is tightly controlled by a vast number of positive and negative regulatory mechanisms. In the steady state, association with inhibitory proteins known as the Inhibitor of NFκB (IκB) family keeps the transcription factor dimers in an inactive state, sequestered in the cytoplasm [8]. The IκB family includes several members, all containing long ankyrin repeat sequences, which mask the Nuclear Localization Signals (NLS) of NFκB transcription factors [9]. The NFκB precursors p100 and p105 also perform this function, with the ankyrin repeat sequences found at their C terminal ends enabling them to act like IκBs [10,11]. Release of NFκB dimers from IκB inhibitory complexes occurs through the action of a third important player in the NFκB pathway; the IκB Kinase (IKK) complex.

Through the ‘classical’ NFκB activation pathway, signals from a wide range of stimuli including proinflammatory cytokines, bacterial and viral components, UV light and oxidative stress can trigger the activation of IKKs [12]. The IKK complex is composed of two catalytic subunits, IKKα and IKKβ, and the regulatory subunit IKKγ (or NEMO). Following the binding of ligands to various cell surface receptors, the IKK complex is activated, phosphorylating IκB at two distinct serine resides; 32 and 36 [13], which causes the ubiquitination and proteolytic degradation of IκB proteins [12]. The removal of IκBα releases transcription factor dimers consisting of p50, RELA and c-Rel subunits, which are free to enter the nucleus, bind to κB sites of target genes and initiate transcription [14]. This pathway facilitates the transcription of genes essential for processes including cell growth, survival, inflammation and adhesion. Furthermore, the RELA/p50 dimer promotes transcription of the gene encoding IκBα, which inactivates the DNA bound transcription factor complex, thus limiting the duration of NFκB signalling in a negative-feedback loop [15,16].

In addition to the classical pathway, another distinct mechanism of NFκB activation referred to as the ‘alternate’ pathway has been described [17]. In this pathway, p100 acts as an IκB, sequestering p52/Rel-b dimers in the cytoplasm [10]. Triggered by stimuli such as BAFF, Lymphotoxin-α and RANKL, the NFκB Inducing Kinase (NIK) activates IKKα, which phosphorylates p100, processing it to p52 [14,18]. The released p52/Rel-b dimers then regulate the transcription of target genes that are particularly important in lymphoid development [19,20]. Though the two pathways of NFκB activation are triggered by different stimuli and regulate the transcription of different target genes, the alternate and classical pathways do not operate in isolation, but are highly interconnected [21,22].

The Role of NFκB in the Development of T Lymphocyte Subsets

The expression pattern of NFκB in the thymus

The generation of the T cell repertoire is a tightly regulated process, which takes place within the thymus. Based on the differential expression of CD4 and CD8 co-receptors, thymic T cell development is generally divided into four stages [23] (Figure 1). The NFκB subunits are expressed throughout thymocyte development, with different dimer complexes playing distinct roles during each developmental stage [24]. Despite some functional redundancy between individual subunits, the study of various knockout and transgenic mouse models has provided significant insights into the role of NFκB during T cell development. The predominant expression of RELA within the thymic cortex suggests a role for this subunit during early T cell development [25]. Due to the embryonic lethality of rela-/- mice [26], the function of RELA in T cell development remains poorly defined, however a recently generated mouse model with a T cell-specific deletion of RELA [27] should provide further insight into the role of this subunit during key stages of thymocyte development. The elevated expression of Rel-b and c-Rel in the thymic medulla suggests their importance at later stages of T cell development [25]. Along with the p52 subunit, the role of Rel-b in signalling via the alternate pathway is strongly associated with the normal function of the thymic stroma [28,29,30].

clinical-cellular-immunology-transcription-factors

Figure 1: The transcriptional control of T cell development and differentiation
Shown is a schematic representation of T cell development along multiple lineages within the thymus (left) and the polarization of distinct T cell subsets in the periphery (right). (A) Highlighted are some of the key transcription factors that direct T cell development along the indicated lineages in the thymus, and promote the phenotype of polarized T helper lineages in the periphery. (B) Shown are the NFκB subunits (and related proteins) for which a cell-intrinsic role in development, differentiation or function has been described. Within the thymus, the CD4-CD8- DN thymocytes develop into CD4+CD8+ DP thymocytes. Positive selection gives rise to a number of thymocyte lineages, including the CD4+ SP, CD8+ SP and nTreg cells. In response to exogenous IL-4 produced by PLZF+ cells, a further innate memory-like CD8+ T cell lineage arises in some mutant and wild-type mouse models. In the periphery, naïve CD4+ T lymphocytes can adopt a number of functional phenotypes including the TFH, TH1, TH2, TH17 and iTreg cells, each producing a distinct cytokine signature. See text for further details.

The role of NFκB in early T cell development

The primary function of NFκB activation during early thymic T cell development is to promote the survival of developing thymocytes. The activation of NFκB ensures the survival of T cell progenitors from TNF-α-induced apoptosis [31], while a role for the p50/RELA heterodimer has been demonstrated in regulating the expression of Jagged and Delta-like ligands, which are members of the Notch family that are crucial for T lineage commitment [32]. The CD4-CD8- Double Negative (DN) thymocytes can themselves be subdivided into four developmental stages on the basis of CD44 and CD25 cell surface expression; stage I (CD44+CD25-), stage II (CD44+CD25+), stage III (CD44-CD25+) and stage IV (CD44-CD25-) [33]. As DN thymocytes progress through these stages, they gradually lose the capacity to enter other lineages, culminating in the expression of a pre-T-cell receptor (TCR) by DN IV cells [34]. Late stage III and early stage IV DN thymocytes show constitutive NFκB activation [35] to promote survival [36]. For example, signalling through the pre-TCR has been shown to activate NFκB [36,37], promoting survival through the transcription of anti-apoptotic target genes such as Bcl-2 [38].

Within DN thymocytes, the NFκB pathway does not operate in isolation, but has been shown to act in concert with other transcription factor families, such as the basic helix-loop-helix transcription factors E2A and HEB, which are critical for thymic T cell development [39]. Studies in thymocytes and T cell lines have suggested that Id1, the inhibitor of E2A and HEB, may mediate up-regulation of NFκB in response to pre-TCR signals - in particular the c-Rel subunit [40]. As a result, the enhanced expression of Id1 in transgenic mice severely impairs T cell development, which is exacerbated by the aberrant activation of NFκB [39,40]. To date, however, the extent and implications of this crosstalk for thymic T cell development remains poorly defined.

The role of NFκB in T cell selection

Based on the signal strength of the TCR and its interaction with selfpeptide- MHC complexes, CD4+CD8+ Double Positive (DP) thymocytes undergo positive and negative selection [41]. Essentially, weak signaling from TCR-peptide/MHC interactions fail to support thymocyte survival (death by neglect), whereas overly strong interactions result in the deletion of potentially autoreactive cells. Only moderate signalling leads to the development of mature CD4+ and CD8+ Single Positive (SP) thymocytes. The precise role of NFκB in mediating thymocyte selection remains controversial. DP thymocytes have been shown to preferentially up-regulate c-Rel and NFκB1-containing dimers [42], but mice lacking individual NFκB subunits have not shown significant defects in T cell selection. An exception are the rel-b-/-, nfκb2-/- and nikaly/aly mice, in which a defective thymic stroma is responsible for impaired negative selection [29,30,43]. The absence of defects in single knockout mutants most likely reflects functional redundancy amongst individual subunits, as other NFκB-specific mouse models including IκB superrepressor and IKK knockout mutants display profound defects in T cell development and selection [44].

Studies in mice expressing a transdominant IκBα, which constitutively represses NFκB signalling, have pointed to a role for NFκB in promoting the survival of DP thymocytes [45-47]. Conversely, others have demonstrated a pro-apoptotic role for NFκB, using TCR transgenic mouse models of negative selection [46]. Furthermore, the expression of a transdominant IκBα was shown to promote the apoptosis of DP thymocytes in response to anti-CD3 treatment in vivo [48]. The level of NFκB inhibition in these different IκB transgenic mouse strains is likely variable and thus may contribute to the observed differences in these mouse models.

Genetic manipulation of the IKK proteins has primarily demonstrated a pro-survival role for NFκB in thymocyte selection. A T cell-specific dominant-negative IKKβ mutation has been shown to promote anti-CD3 induced apoptosis [49], while the expression of a kinase-deficient IKKβ was found to impair the generation of CD8 SP thymocytes [50]. In further support of an anti-apoptotic role for NFκB, it was shown that negative selection triggers the expression of an NFκB inhibitor [51]. Intriguingly, the conditional deletion of IKKβ in the T cell lineage does not significantly impact the development of conventional T cells, but impairs the generation of both NKT and T regulatory (Treg) cell lineages [50], suggesting that the functions of NFκB during the development of conventional versus non-conventional thymocyte lineages differ significantly.

While the precise role of NFκB in negative selection remains subject for debate, a recent study has suggested that NFκB may contribute to the orchestration of selection thresholds [52]. Low affinity TCR signalling may trigger low levels of NFκB activation to promote survival, while TCR affinity above a defined threshold triggers high levels of NFκB signalling, to provide opposing pro-apoptotic responses [52]. Surprisingly, increased NFκB activity was also shown to skew thymocyte selection in favor of conventional CD8+ T cells, but was dispensable for the development of CD4+ T cells [47,52].

Lineage commitment of conventional and non-conventional T cells

The thymic microenvironment promotes the development of DP thymocytes along multiple lineages (see Figure 1). Whilst the role of NFκB in promoting DP thymocyte selection appears to be specific for the CD8+ but not the CD4+ T cell lineage [47,52]. It is also apparent that NFκB signalling is important in regulating the development of CD4+ Treg cells [53,54], NKT cells [55,56] and a unique population of memory-like CD8+ T cells [57].

Although the precise function of NFκB in this contexts is yet to be fully resolved, the normal development of NKT cells requires the presence of Rel-b in the thymic stroma [55,58], while the NFκB1 subunit has been shown to play a thymocyte-intrinsic role in NKT cell development [55,56]. A critical role for c-Rel has been demonstrated in the thymic development of Treg cells [53,59-62]. The c-Rel subunit is highly expressed within Treg cells [53] and c-rel-/- mice show a T cell intrinsic defect in Treg development [53,54]. This is in contrast with findings from nfκb1-/- mice, which show normal Treg development, suggesting that c-Rel and NFκB1 play distinct roles during lineage commitment [54]. Furthermore, these findings support a role for the classical NFκB pathway in Treg development, initially demonstrated in the IKΚβ knockout mutants, which also show defects in the development of Treg cells [50]. The absence of alternate pathway components Rel-b and NFκB2 has been shown to reduce Treg development, though this arises through disruption of the thymic stroma, rather than thymocyteintrinsic defects. The function of NIK within thymic stromal cells is also required to promote T cell tolerance [30], yet in addition to this developmental role evidence is accumulating that NIK function is required in Treg cells [19,63,64]. Peripheral T cell function is largely normal in the absence of NIK [19], and although NIK-deficient mice have fewer peripheral Treg cells their suppressive function is maintained [63]. Primarily as a result of reduced Treg function, the overexpression of NIK specifically within CD4+ T cells has been reported to induce lethal autoimmunity [64]. These studies suggest a role for NIK in the regulation of Treg cells, through mechanisms that are yet to be defined.

The development and function of memory-like CD8 SP thymocytes

Recently, the study of mouse models deficient in critical transcription factors or kinases such as CBP, Id3, Itk, Rlk and Klf2, has revealed a unique T cell lineage termed innate or ‘memory-like’ CD8+ T cells [65-69]. In contrast to peripheral memory T cells that normally develop in response to foreign antigens, these T cells acquire their memory characteristics during development in the thymus. They are primarily restricted to the CD8 lineage, and posses the classic hallmarks of peripheral memory T cells, which includes the elevated expression of memory markers (CD44, CD122, CXCR3 and Ly6C) [66], and the ability to mount a rapid and robust IFN-γ response when stimulated (Table 1). The T-box transcription factor, Eomesodermin, which promotes a pattern of gene expression that is typical of memory CD8+ T cells [70], is also highly elevated in these memory-like CD8+ T cells.

Mouse Models Common features Unique features References
itk-/-; itk-/-rlk-/-
cbp-/-; klf2-/-
β-catenin-/-
CD8αβ+
TCRαβ+
CD44hi
Ly6c+
CD122+
CXCR3+
Eomes+
Rapid TCR mediated IFN-γ production
Altered disease susceptibility*
Reduced CD5 expression*
IL-15 dependent*
Selected by MHC class Ia or Ib on haematopoietic cells
Development of memory-like T cells is SAP dependent*
Elevated IL-4 producing PLZF+ cell population
[164] [165,166]
[167,168]
[67,72,169] [170] [66]
id3-/- Selection by TECs
Development of memory-like T cells is SAP-dependent
Elevated CD5 expression
Susceptible to Sjogren’s disease
Elevated IL-4 producing PLZF+ cell population
[69] [171,172]
[69]
nfκb1-/- IL-15 independent
No evidence of an elevated PLZF+ cell population
Selected by MHC class Ia or Ib on haematopoietic cells
Elevated CD5 expression
Reduced intrathymic Sirpαhi DCs
[57]
Balb/c KLF13 dependent
Altered disease susceptibility
Wild-type mouse strain
[74]
[75,76]

* Denotes features to be confirmed in CBP, KLF2 and β-Catenin deficient mice.

Table 1: Distinguishing features of memory-like CD8+ T cells in wild-type and mutant mouse models.

Aside from their effector/memory properties, memory-like CD8 SP cells are distinct from CD1d-restricted NKT, intraepithelial CD8+ TCRαβ+ and TCRγδ+ T cells [71]. Like conventional CD8 SP thymocytes, they express the CD8αβ co-receptor rather than CD8αα homodimers and utilize a diverse TCR repertoire (Table 1), however the rules of T cell selection appear to vary between conventional and memory-like CD8+ T cells. Unlike conventional CD8+ T cells, which are selected via MHC class I expressing TECs, memory-like CD8+ T cells are positively selected through class Ia or Ib expressing haematopoietic cells [72,73]. The finding that id3-/- memory-like CD8 SP thymocytes are positively selected by MHC class I expressing TECs [69] suggests that selection by haematopoietic cells may not be a critical requirement for the acquisition of memory properties by developing thymocytes. While the process of selecting memory-like thymocytes requires further investigation, a recent advance in the field was the finding that developing CD8 SP thymocytes from mouse models, such as the itk-/- and klf2-/- mutants, acquire memory characteristics through exposure to IL-4 produced by NKT or γδ T cells that express the transcription factor PLZF [66].

Our recent finding demonstrated that mice lacking NFκB1 develop a unique population of CD8 SP thymocytes exhibiting memory characteristics [57]. Development of these memory-like thymocytes is independent of the IL-4 producing PLZF+ population. Instead, the acquisition of nfκb1-/- memory-like CD8 SP thymocytes coincided with changes in T cell selection, including reduced efficiency of negative selection and positive selection by either MHC class Ia or Ib molecules presented by haematopoietic cells [57]. Whilst this study highlights the importance of NFκB1 in preventing the development of CD8 SP thymocytes with memory characteristics, a potential role for IL-4 could not be totally excluded. Subsequent work will need to consider the study of CD8+ T cells in il-4-/- nfκb1-/- double knockout mice, and examine the scenario of altered IL-4 receptor signalling on developing nfκb1-/- thymocytes.

An important issue that remains to be addressed is whether memory-like CD8 SP thymocytes are disease promoting, or have the capacity to provide protective immunity, like NKT cells. Firstly, the finding that memory-like CD8+ T cells also arise in the thymi of Balb/c mice [74] supports the physiological relevance of this T cell population and indicates that wild-type mice can harbor these T cells without detrimental effects. Consistent with some mutant mouse models, memory-like CD8+ T cells in Balb/c mice are promoted by IL-4 secreting NKT cells with a critical role for the transcription factor KLF13 [74]. Balb/c mice have a natural resistance to intracellular pathogens such as the adenovirus type 1 [75] and measles virus [76], and it is possible that memory-like T cells may contribute to the resistance to these pathogens [74].

An equivalent population of human memory-like CD8+ T cells is yet to be unequivocally identified, however in some settings these T cells seem to be promoted. For instance, a population of stem cell-like memory CD8+ T cells has been identified in humans [77]. Furthermore, loss of function Itk mutations have been observed [78], and like the murine counterpart [67] Itk deficiency is associated with elevated Eomes+ CD8+ T cells [79]. Itk deficiency has also been linked with EBV-associated lymphoproliferative disease [76], raising the possibility that memory-like T cells are associated with aberrant lymphoproliferative disease. A subpopulation of memory-like CD8+ T cells has been shown to play a critical role in lymphopaenic postchemotherapy patients heavily susceptible to viral infection [80]. These cells, which resemble the memory-like population in mice, have the capacity to survive exposure to chemotherapy drugs and efflux rapidly to restore immunity in such individuals [80]. Memory-like CD8+ T cells have also been shown to display significant anti-tumour responses in humans [77], suggesting they may be important for the generation of tumour vaccines or T cell therapies. As we gain further insight into the transcriptional programs that drive the development of memorylike CD8+ T cells, it will be crucial to determine whether these T cells initiate or contribute to particular lymphoproliferative diseases, or whether this unique T cell population holds significant therapeutic potential.

NFκB in Peripheral T cell Function

For optimal T cell immunity, the adaptive immune system maintains a constant pool of naïve CD4+ and CD8+ T cells, from which long-lived memory T cells are produced. Naïve T cells rarely divide and contain low levels of p50 homodimers in the nucleus [81]. Homodimer binding is believed to repress expression of IL-2, the major cytokine involved in autocrine pro-proliferative signalling [81]. In response to various pathogens including microbes and viruses, TCR and costimulatory signals induce the activation and proliferation of antigen-specific T cells in the periphery. NFκB signalling regulates the activation and proliferation of naïve T cells, both in a T cell-intrinsic manner [82-86], and through the control of T cell-extrinsic processes, such as cytokine production by APCs [82,87-90].

As T cells shift from resting to activated, the profile of NFκB transcription factors in the nucleus changes in a biphasic series of events, which are well described in vitro [91,92]. Upon T cell receptor engagement, p50/RELA heterodimers accumulate in the nucleus [81] followed by c-Rel containing dimers, which up-regulate the expression of IL-2 [93,94]. This influx of NFκB transcription factors promotes proliferation and survival, with inhibition of NFκB signalling shown to impair proliferation and increase apoptosis of T cells, following mitogenic stimulation [45,48]. However, the outcome of activation is dependent on the type and strength of stimuli, as well as the specific cell type activated. The IKΚβ subunit is absolutely required for the generation of optimal antigen-specific T cell responses in vivo [95]. The deletion of IKΚβ specifically within T lymphocytes was shown to reduce proliferation following antigen-specific restimulation, resulting in sub-optimal T cell dependent B cell help and impaired homeostatic expansion of T lymphocytes [95]. However IKΚβ is dispensable for T cell activation and proliferation in response to strong stimuli [50]. This is demonstrated by the ability of IKΚβ-deficient T cells to proliferate normally in response to polyclonal TCR stimulation, likely the result of residual signalling via remaining IKKα/NEMO complexes [50].

Both the classical and alternate NFκB pathways are important for T cell activation and function in the periphery [20], though the former is most heavily implicated in T cell-intrinsic functions. For example, components of the classical pathway are essential for regulating the progression of activated T cells through distinct phases of the cell cycle [93,96]. Both c-Rel and RELA are required to induce the progression of activated T cells through G0 to G1, and promote c-Myc-induced proliferation following TCR stimulation [85]. TCR-induced cell cycle entry and survival is also dependent on the activity of NFκB1 and c-Rel [97]. These subunits serve redundant functions, with defects in T cell proliferation and survival observed in nfκb1-/- c-rel-/- compound mutants, which do not develop in either nfκb1-/- or c-rel-/- mice [97].

T cell polarization

The CD4+ T lymphocytes can adopt a number of functional phenotypes, each producing a unique cytokine signature (Figure 1). The key T-helper subsets include the Th1, Th2, Th17 and T-follicular helper (TFH) cells, and distinct T cell intrinsic and extrinsic factors regulate their polarization and function. The precise function of NFκB in this process is still being elucidated. However, individual NFκB subunits have been shown to preferentially regulate the Th1 versus Th2 response [98]. In particular, c-Rel plays a key role in promoting the polarization of Th1 cells [82], and the loss of this subunit leads to defective Th1 responses via multiple mechanisms [82]. These include the reduced production of IL-12 by APCs [89], which confers disease resistance to Experimental Autoimmune Encephalomyelitis (EAE) in c-rel-/- mice [82]. The c-Rel subunit also plays critical T cell-intrinsic roles during Th1 cell differentiation. When cultured under Th1 polarizing conditions, c-Rel deficient CD4+ T cells show reduced IFN-γ production [83] but normal Tbet expression [82]. In c-rel-/- mice, this defect in the expansion of IFN-γ+ Th1 cells results in enhanced susceptibility to T. gondii infection [86]. Similarly, the absence of Rel-b leads to impaired IFN-γ production in response to T. gondii infection in rel-b-/- mice [87]. Rel-b-deficient CD4+ T cells display defective Th1 differentiation and IFN-γ production in vitro, and reduced expression of both Tbet and STAT4 transcription factors has been reported in rel-b-/- Th1-polarized cells [99].

Conversely, the NFκB1 subunit appears to preferentially regulate Th2-type responses. A number of in vitro studies have suggested a role for NFκB1 p50 in promoting GATA3 expression under Th2 polarizing conditions [88,99] and the p50 subunit, along with its DNA-binding partner Bcl-3, have been demonstrated to participate directly in the transactivation of GATA3 [99]. This finding translates in vivo to the prevention of airway inflammation in nfκb1-/- mice, due to impaired production of the Th2 cytokines IL-4, IL-5 and IL-13 [88]. Furthermore, the proteolysis of p105 is critical for the optimal proliferation of CD4+ T cells in response to TCR activation, and in the generation of sufficient Treg and memory T cell numbers [96].

The Th17 cells are a CD4+ T-helper subset distinct from both Th1 and Th2 cells, which are characterized by the production of IL- 17 [100]. These cells are linked with inflammatory-mediated tissue injury, in autoimmune conditions such as rheumatoid arthritis and multiple sclerosis [101]. Though the role of NFκB in this cell subset is currently unclear, recent evidence has suggested that c-Rel activity may be essential for promoting Th17 cell development [102]. In the absence of c-Rel, the generation of Th17 cells is severely reduced, both in vitro and in response to disease models [102]. The impaired generation of Th17 cells in the absence of c-Rel may therefore be an important mechanism underlying the observed resistance of c-rel-/- mice to EAE [102]. The RELA subunit has also been shown to promote Th17 cell generation, in this case by enhancing the production of inflammatory cytokines by DCs [90]. Further studies have supported a critical role for the classical pathway in Th17 cell differentiation, demonstrating that c-Rel and RELA can bind and activate two distinct Rorg promoters, to control RORγt expression and drive Th17 cell differentiation [103]. The alternate NFκB pathway is also implicated in the differentiation of Th17 cells [104]. Through cell intrinsic mechanisms, the absence of NIK has been shown to impair the production of Th17 cell-associated cytokines and confer resistance to EAE [104]. More recently, the role of NIK in promoting Th17 responses has since been attributed to DCmediated mechanisms [105].

Another T helper subset known as Follicular T-helper (TFH) cells play an essential role in regulating T cell dependent-B cell responses and the formation of germinal center (GC) reactions [106]. Whilst recent studies have contributed significantly to our understanding of TFH cells, little is currently known about the role of NFκB in this particular subset. Interestingly, a role for c-Rel has recently been described in promoting the production of IL-21 by CD4+ T lymphocytes [107], a cytokine which is critical for the expansion and differentiation of TFH cells [108,109]. As a result, c-rel-/- mice display a defect in TFH cell development, which is rescued by the administration of exogenous IL-21 [107]. A T cell extrinsic role for the alternate NFκB signalling pathway in TFH cell differentiation has also been suggested, with NIK found to regulate the expression of ICOSL in B cells [110]. While Bcl6, a vital transcription factor for the differentiation and function of TFH cells has been shown to act as a direct inhibitor of NFκB1 expression [111]. Considering the emerging association of TFH cells with autoimmune disease [106], it will be important to further define the role of NFκB in the development and function of this cell subset.

To date, our understanding of NFκB activation during T cell polarization has been complicated by the dual roles of NFκB in mediating both T cell intrinsic and extrinsic processes. Future studies examining mice with T cell specific deletions of NFκB proteins will shed further light on the role of NFκB in regulating T helper cell differentiation. Findings to date suggest that both the classical and alternate pathways of NFκB signalling are important in regulating T cell polarization. However, the classical pathway may be the primary regulator of T cell intrinsic mechanisms, including the direct regulation of critical transcription factors for polarization such as GATA3 [99] and RORγt [103]. Furthermore, little is currently known about the role of NFκB in the more recently identified T helper subsets, the Th17 and TFH cells. The association of these subsets with human disease and their status as potential therapeutic targets makes this an important area for future research.

T cell malignancies

The first suggestion of a link between NFκB and oncogenesis was made with the cloning of nfκb1 in 1990 [112,113]. These initial studies revealed sequence homology with v-Rel; a viral oncogene known to cause aggressive lymphoid malignancies [113]. There are a number of ways in which dysregulated NFκB signalling can contribute to oncogenesis. In the face of excessive or inappropriate activation, the many growth promoting and anti-apoptotic target genes of NFκB can aid the growth and survival of malignant cells [114]. Furthermore, NFκB regulates the expression of adhesion molecules, chemokine receptors, and matrix metalloproteinases; all of which are implicated in the invasion and metastasis of tumour cells [114,115]. Importantly, NFκB signalling is well recognized as a critical link between inflammation and tumour development [3]. Up to 20% of all human malignancies are believed to be the result of this process, wherein chronic inflammation leads to the neoplastic transformation of cells [3,116].

Activating mutations in NFκB subunits occur only rarely in human T cell malignancies however [117], the most common of these being genetic alterations in c-Rel, the cellular counterpart to v-Rel [118]. Amplification of the c-rel gene, which correlates with elevated nuclear c-Rel protein [119], has been identified in a small number of T cell malignancies, in particular T cell lymphomas [118-120]. While mutation of the NFκB2 gene has been identified in approximately 2% of T cell malignancies including Acute Lymphocytic Leukemia (ALL) and cutaneous T cell lymphomas [117]. The majority of these rearrangements involve deletion of the inhibitory C terminal region of p100, producing a truncated protein that confers constitutive p52 activity [121]. Genetic alteration of the RELA, Rel-b or NFκB1 subunits has been identified only in sporadic cases of ALL and NHL.

A much greater proportion of T cell malignancies have been found to display constitutive or enhanced NFκB activation, without genetic alteration [122-124]. This is believed to occur indirectly, as a result of aberrant upstream signalling or a loss of regulatory mechanisms [125]. Studies have identified an important role for constitutive NFκB signalling in promoting the growth, survival and tumourigenicity of many malignant cell types [124,126-128]. Numerous studies have also begun to investigate the types and frequency of NFκB polymorphisms, and evaluate the association with tumourigenesis [129,130]. Although this association remains unclear [130], and the full implications of NFκB polymorphisms in tumourigenesis are yet to be determined, these studies may provide a means for tailored therapies and diagnostics in the future [129,130].

Complex Interactions with Other Signalling Pathways

Recent years have seen our understanding of intracellular signalling grow; from a series of defined pathways to a complex signalling network. It has become clear that signalling ‘crosstalk’ is a critical feature of T cell activation, which allows the integration of multiple stimuli and the precise modulation of intracellular responses. Studies have begun to identify the points at which parallel signalling pathways converge with NFκB. Of these, the most thoroughly investigated is the IKK complex. Once believed to be highly specific for the IκB proteins, it has since become clear that the IKK complex also targets upstream components of NFκB as well as a number of unrelated pathways.

IKKα

IKKα plays an important role in phosphorylating non-NFκB/IκB substrates, and the IKKα subunit itself has a NLS. As a result, many of IKKα’s non-NFκB targets are nuclear, including histone H3 [131] and other proteins involved in cell growth and metastasis [132,133]. One potential target of IKKα with clear implications for T cell function is IL-17A [134]. It was recently found that during differentiation of Th17 cells, IKKα associated with the Il17a locus to promote transcription and commitment to the Th17 cell lineage [134]. However the identity of nuclear targets and the mechanisms of regulation by IKKα are still poorly defined and little is known about the specific cell types and contexts in which they operate.

The IKKα subunit is also implicated in the extensive crosstalk between NFκB and Notch signalling. The reciprocal regulation of these two pathways involves a complex series of interactions [135]. Stimulation of T cell lines with Notch ligand activates NFκB by promoting the direct interaction of Notch1 with IKKα [136]. In primary murine T cells, direct binding has been demonstrated between Notch1 and p50/ c-Rel complexes [137]. Though our understanding of this crosstalk is limited, the activation of NFκB by the Notch pathways has important implications for T cell acute lymphoblastic leukemias (T-ALL), where Notch signalling is commonly activated [138]. Furthermore, crosstalk between IKKα and Notch1 has recently been identified as a critical pathway for inflammatory-mediated tumourigenesis [139].

A further example, which has been a topic of debate for some time, is the crosstalk between NFκB and the tumour-suppressor protein p53. The NFκB and p53 transcription factors are activated in response to many of the same stimuli and present a critical crossroads for cell fate, determining whether the outcome of stimulation will be proliferation or cell death. Competition for binding to the transactivator CBP has long been believed to mediate crosstalk between these two transcription factor pathways [140]. This competition may be regulated by IKKα, which can phosphorylate CBP to induce preferential binding with NFκB over p53, thus enhancing NFκB- and repressing p53-dependent transcription [141]. Another mechanism linking the alternate NFκB pathway and p53 has also been suggested, via direct regulation of p53 transcriptional activity by NFκB2 (p52). The p53 transcription factor may recruit p52 to its target regions, where in turn p52 can either repress or activate p53 [142]. However, more recent studies examining the response of primary murine thymocytes to a range of stress-induced stimuli have failed to demonstrate functional crosstalk between NFκB and p53 [143]. These conflicting findings highlight the difficulties associated with the study of signalling crosstalk. The effect of a given intervention will inherently be obscured by defects in the central signalling pathway, and thus in many cases clear biological relevance of signalling crosstalk is yet to be demonstrated.

These examples are only some of the pathways in which non-NFκB functions for IKKα have been described. Overall, it is clear that the physiological implications of signalling crosstalk with IKKα in a cell type and context specific view are yet to be fully realized. Certainly it may hold implications for various malignancies, where cell fate decisions are critical for oncogenic transformation and progression. Moreover, it will be intriguing to determine how the link between IKKα and Notch signalling impacts on key aspects of normal T cell development and function in vivo, in particular those processes regulated via the alternate NFκB signalling pathway.

IKKβ and Tpl2

Perhaps the best example of signalling crosstalk with the NFκB pathway is the complex relationship between NFκB and Tpl2. Tpl- 2 is a Mitogen-Activated Protein Kinase Kinase Kinase (MAP3K), which activates ERK in response to immune and inflammatory signals [144]. In the steady state Tpl2 forms a stable and inactive complex with p105, which is required to prevent the degradation of Tpl2 [145]. Consequently, the levels of Tpl2 found in nfκb1-/- (p105 deficient) cells are severely reduced [145]. Moreover, binding with p105 prevents substrate access to the kinase domain of Tpl2, allowing p105 to function as an inhibitor of MEK kinase activity [146]. An important consequence of IKKβ activation is therefore the release of Tpl2. IKKβ can induce proteolysis of p105, which leads to the release and activation of Tpl2, making IKKβ and p105 crucial regulators of both NFκB and MAPK signalling. Tpl2 is not the sole link between these pathways, as IKKβ can also phosphorylate Dok1, a negative regulator of cell growth, which leads to the inhibition of ERK activation [147]. These opposing effects of IKK activation on the MAPK pathway clearly highlight the stringent regulation required for ERK activation, and the contextdependent nature of signaling crosstalk with NFκB.

With regard to T cell activation, the over-expression of Tpl2 was initially found to induce the production of IL-2 in Jurkat T cells [148]. This induction is suggested to involve activation of the alternate NFκB pathway via the MAP3K NIK. Similar studies also highlight the possibility that Tpl2 may regulate NFκB signalling by inducing p105 proteolysis [149] and/or by modulating the transactivation potential of RELA [150]. Considering the limitations of over-expression systems, delineating the physiological relevance of NFκB regulation by Tpl2 is crucial. In response to TCR stimulation in vitro, CD4+ T cells deficient in Tpl2 have been shown to produce normal levels of IL-2 [96,151]. No overt phenotypes have been observed in the tpl2-/- mice unless challenged by antigen [151]. In particular, T cell development in the thymus of these mutants is not impaired [151], which may reflect a redundant role for Tpl2 within the ERK signalling pathway, as the importance of ERK1/2 [152] and the fellow MAP3K Raf [153] in thymocyte development and selection are both well recognized.

The study of Tpl2 deficient mice has suggested a critical role for Tpl2 in the differentiation of Th1 cells. The expression of Tpl2 mRNA is highest in Th1, as compared with Th2-polarized cells [154], while tpl2-/- CD4+ T cells cultured under Th1 polarizing conditions show reduced IFN-γ production [154], and display marginally reduced Tbet and STAT4 expression in response to TCR stimulation [155]. Furthermore, resistance to the intracellular parasite Toxoplasma gondii, which is critically dependent on the optimal production of IFN-γ, is impaired in tpl2-/- mice [154]. The investigation of Tpl2 function in vivo is complicated by the study of an independently generated and phenotypically distinct Tpl2-deficient mouse model that showed a heavily skewed Th1 response against Leishmania major infection [156]. To date these discordant results are yet to be resolved and may reflect numerous factors including the background strain, infection model, and method of generation. Furthermore, the role of Tpl2 in the differentiation of other T helper subsets including the Th17, TFH and Treg cells remains to be determined conclusively.

Like the NFκB proteins, Tpl2 has been identified as a potent experimental oncogene [157]. Early studies described the tpl2 gene as a target of provirus insertion in retrovirus MoMuLV-induced rodent T cell lymphoma [158]. In such cases, viral insertion in the last intron of tpl2 results in a C-terminal truncation of the Tpl2 protein. Similarly truncated forms of Tpl2 were subsequently linked with large cell lymphoblastic T cell lymphomas in mice [159]. Intriguingly however, overexpression of wild-type tpl2 in transgenic mice fails to promote lymphomagenesis [159], emphasizing an important effect of C terminal truncation that remains to be fully understood. Tpl2 has also been found to act as a tumour suppressor in certain contexts. For example, tpl2-/- mice bred onto a TCR transgenic, MHC Class I-restricted background have been shown to develop T cell lymphomas [160]. This appears to be the result of defective induction of CTLA-4 following TCR stimulation, leading to an exaggerated CD8+ T cell response [160].

Despite these murine studies, the screening of human malignancies has to date revealed relatively few cases of Tpl2 mutations, with the exception of a small number of large granular T cell neoplasias [161]. Nonetheless a role for altered expression and/or function of Tpl2 in human malignancies, mediated by factors including inflammation and NFκB activation, cannot be discounted. A detailed examination of Tpl2 expression and function in human disease has not yet been conducted, though there is evidence that tpl2 mRNA levels may be elevated in some forms of malignancy and reduced in others [162].

Importantly, the consequences of linking NFκB and MAPK activation remain largely unknown, and infinitely complex. It is important to note that studies involving the manipulation of NFκB pathways, in particular the IKKβ/p105 components must be examined with the knowledge that Tpl2 and MAPK activation are likely altered. Furthermore, a key question remains regarding whether Tpl2 functions physiologically only in the regulation of MAPK signalling, or if it also phosphorylates other proteins and thus other signalling pathways, including NFκB [150]. Taken together, the examples discussed herein highlight a means through which the IKK complex may influence immune function and oncogenesis, independently of NFκB, by mediating crosstalk with other signalling pathways. And conversely, suggests the possibility that modulation of NFκB by unrelated signalling pathways may be a critical factor in certain cell types and contexts. Teasing apart such complex interactions, and determining how they function both in physiological immune processes and in the context of disease is a difficult task. What is clear, however, is that determining the functional implications of crosstalk with the NFκB pathways, and identifying the full complement of IKK targets is an important research goal, particularly in light of the ongoing efforts to develop targeted therapeutics.

Perspectives

The complex nature of NFκB signalling presents significant challenges for studying the role of this transcription factor in T lymphocytes. These challenges include the subunit redundancy of individual family members, and the concurrent functions of NFκB in APCs and other cell types, which influence T cell development and function. However, the application of emerging technologies and newer mouse models should help to overcome many of these issues, and provide answers to some of the outstanding questions. The role of NFκB in the development of non-conventional T cell lineages remains unclear, in particular regarding the development of memory-like CD8+ T cells. Likewise, our understanding of NFκB function in the differentiation and function of peripheral T helper subsets including the Th17 and TFH cells is limited. Furthermore, there is still much to be learnt about the crosstalk mechanisms that operate between NFκB and other intracellular signalling pathways, and how signalling crosstalk functions physiologically.

Nonetheless, significant insights have been gained over the years into the role of NFκB during various stages of T cell development, and in the function of multiple T cell lineages. It is abundantly clear that NFκB plays vital roles in many physiological processes, and that the consequences of dysregulated NFκB signalling are many and varied. It is for this reason that NFκB remains an attractive therapeutic target. However, its critical role in immune function is also the biggest obstacle facing NFκB-targeted therapies. The inhibition of upstream NFκB components, such as the IKK complex, can have severe and detrimental effects on immune function [163]. To minimize these risks, future research will seek to develop selective inhibitors of NFκB subunits, as well as methods of modulating NFκB function within specific cell types or contexts. A further goal will be to target only NFκB-specific functions, while leaving essential mechanisms of signalling crosstalk with other intracellular pathways intact. Though a challenging task, the importance of NFκB in T cell development and function ensures the endeavor to understand it will be worthwhile.

Acknowledgements

The authors are grateful to the Victorian Operational Infrastructure Support Program. E d V is supported by an Australian Postgraduate Award and R G is funded by the National Health and Medical Research Council (NHMRC project grants 356219 and 603713).

References

  1. Sen R, Baltimore D (1986) Multiple nuclear factors interact with the immunoglobulin enhancer sequences. Cell 46: 705-716.
  2. Gilmore TD (2006) Introduction to NF-kappaB: players, pathways, perspectives. Oncogene 25: 6680-6684.
  3. DiDonato JA, Mercurio F, Karin M (2012) NF-κB and the link between inflammation and cancer. Immunol Rev 246: 379-400.
  4. Hoffmann A, Natoli G, Ghosh G (2006) Transcriptional regulation via the NF-kappaB signaling module. Oncogene 25: 6706-6716.
  5. Palombella VJ, Rando OJ, Goldberg AL, Maniatis T (1994) The ubiquitin-proteasome pathway is required for processing the NF-kappa B1 precursor protein and the activation of NF-kappa B. Cell 78: 773-785.
  6. Chen FE, Huang DB, Chen YQ, Ghosh G (1998) Crystal structure of p50/p65 heterodimer of transcription factor NF-kappaB bound to DNA. Nature 391: 410-413.
  7. Hayden MS, Ghosh S (2008) Shared principles in NF-kappaB signaling. Cell 132: 344-362.
  8. Baeuerle PA, Baltimore D (1988) I kappa B: a specific inhibitor of the NF-kappa B transcription factor. Science 242: 540-546.
  9. Jacobs MD, Harrison SC (1998) Structure of an IkappaBalpha/NF-kappaB complex. Cell 95: 749-758.
  10. Dobrzanski P, Ryseck RP, Bravo R (1995) Specific inhibition of RelB/p52 transcriptional activity by the C-terminal domain of p100. Oncogene 10: 1003-1007.
  11. Liou HC, Nolan GP, Ghosh S, Fujita T, Baltimore D (1992) The NF-kappa B p50 precursor, p105, contains an internal I kappa B-like inhibitor that preferentially inhibits p50. EMBO J 11: 3003-3009.
  12. Karin M, Ben-Neriah Y (2000) Phosphorylation meets ubiquitination: the control of NF-[kappa]B activity. Annu Rev Immunol 18: 621-663.
  13. Chen Z, Hagler J, Palombella VJ, Melandri F, Scherer D, et al. (1995) Signal-induced site-specific phosphorylation targets I kappa B alpha to the ubiquitin-proteasome pathway. Genes Dev 9: 1586-1597.
  14. Lawrence T (2009) The nuclear factor NF-kappaB pathway in inflammation. Cold Spring Harb Perspect Biol 1: a001651.
  15. Sun SC, Ganchi PA, Ballard DW, Greene WC (1993) NF-kappa B controls expression of inhibitor I kappa B alpha: evidence for an inducible autoregulatory pathway. Science 259: 1912-1915.
  16. Natoli G, Chiocca S (2008) Nuclear ubiquitin ligases, NF-kappaB degradation, and the control of inflammation. Sci Signal 1: pe1.
  17. Senftleben U, Cao Y, Xiao G, Greten FR, Krähn G, et al. (2001) Activation by IKKalpha of a second, evolutionary conserved, NF-kappa B signaling pathway. Science 293: 1495-1499.
  18. Xiao G, Harhaj EW, Sun SC (2001) NF-kappaB-inducing kinase regulates the processing of NF-kappaB2 p100. Mol Cell 7: 401-409.
  19. Yin L, Wu L, Wesche H, Arthur CD, White JM, et al. (2001) Defective lymphotoxin-beta receptor-induced NF-kappaB transcriptional activity in NIK-deficient mice. Science 291: 2162-2165.
  20. Vallabhapurapu S, Karin M (2009) Regulation and function of NF-kappaB transcription factors in the immune system. Annu Rev Immunol 27: 693-733.
  21. Basak S, Shih VF, Hoffmann A (2008) Generation and activation of multiple dimeric transcription factors within the NF-kappaB signaling system. Mol Cell Biol 28: 3139-3150.
  22. Basak S, Kim H, Kearns JD, Tergaonkar V, O'Dea E, et al. (2007) A fourth IkappaB protein within the NF-kappaB signaling module. Cell 128: 369-381.
  23. Takahama Y (2006) Journey through the thymus: stromal guides for T-cell development and selection. Nat Rev Immunol 6: 127-135.
  24. Gerondakis S, Siebenlist U (2010) Roles of the NF-kappaB pathway in lymphocyte development and function. Cold Spring Harb Perspect Biol 2: a000182.
  25. Sen J, Venkataraman L, Shinkai Y, Pierce JW, Alt FW, et al. (1995) Expression and induction of nuclear factor-kappa B-related proteins in thymocytes. J Immunol 154: 3213-3221.
  26. Beg AA, Sha WC, Bronson RT, Ghosh S, Baltimore D (1995) Embryonic lethality and liver degeneration in mice lacking the RelA component of NF-kappa B. Nature 376: 167-170.
  27. Powolny-Budnicka I, Riemann M, Tänzer S, Schmid RM, Hehlgans T, et al. (2011) RelA and RelB transcription factors in distinct thymocyte populations control lymphotoxin-dependent interleukin-17 production in γδ T cells. Immunity 34: 364-374.
  28. Zhang X, Wang H, Claudio E, Brown K, Siebenlist U (2007) A role for the IkappaB family member Bcl-3 in the control of central immunologic tolerance. Immunity 27: 438-452.
  29. Weih F, Carrasco D, Durham SK, Barton DS, Rizzo CA, et al. (1995) Multiorgan inflammation and hematopoietic abnormalities in mice with a targeted disruption of RelB, a member of the NF-kappa B/Rel family. Cell 80: 331-340.
  30. Kajiura F, Sun S, Nomura T, Izumi K, Ueno T, et al. (2004) NF-kappa B-inducing kinase establishes self-tolerance in a thymic stroma-dependent manner. J Immunol 172: 2067-2075.
  31. Igarashi H, Baba Y, Nagai Y, Jimi E, Ghosh S, et al. (2006) NF-kappaB is dispensable for normal lymphocyte development in bone marrow but required for protection of progenitors from TNFalpha. Int Immunol 18: 653-659.
  32. Bash J, Zong WX, Banga S, Rivera A, Ballard DW, et al. (1999) Rel/NF-kappaB can trigger the Notch signaling pathway by inducing the expression of Jagged1, a ligand for Notch receptors. EMBO J 18: 2803-2811.
  33. Godfrey DI, Kennedy J, Suda T, Zlotnik A (1993) A developmental pathway involving four phenotypically and functionally distinct subsets of CD3-CD4-CD8- triple-negative adult mouse thymocytes defined by CD44 and CD25 expression. J Immunol 150: 4244-4252.
  34. Hoffman ES, Passoni L, Crompton T, Leu TM, Schatz DG, et al. (1996) Productive T-cell receptor beta-chain gene rearrangement: coincident regulation of cell cycle and clonality during development in vivo. Genes Dev 10: 948-962.
  35. Feuillard J, Mémet S, Goudeau B, Lilienbaum A, Schmidt-Ullrich R, et al. (2000) In vivo identification of lymphocyte subsets exhibiting transcriptionally active NF-kappaB/Rel complexes. Int Immunol 12: 613-621.
  36. Voll RE, Jimi E, Phillips RJ, Barber DF, Rincon M, et al. (2000) NF-kappa B activation by the pre-T cell receptor serves as a selective survival signal in T lymphocyte development. Immunity 13: 677-689.
  37. Jimi E, Phillips RJ, Rincon M, Voll R, Karasuyama H, et al. (2005) Activation of NF-kappaB promotes the transition of large, CD43+ pre-B cells to small, CD43- pre-B cells. Int Immunol 17: 815-825.
  38. Mandal M, Borowski C, Palomero T, Ferrando AA, Oberdoerffer P, et al. (2005) The BCL2A1 gene as a pre-T cell receptor-induced regulator of thymocyte survival. J Exp Med 201: 603-614.
  39. Kim D, Xu M, Nie L, Peng XC, Jimi E, et al. (2002) Helix-loop-helix proteins regulate pre-TCR and TCR signaling through modulation of Rel/NF-kappaB activities. Immunity 16: 9-21.
  40. Yang Y, Liou HC, Sun XH (2006) Id1 potentiates NF-kappaB activation upon T cell receptor signaling. J Biol Chem 281: 34989-34996.
  41. Hogquist KA (2001) Signal strength in thymic selection and lineage commitment. Curr Opin Immunol 13: 225-231.
  42. Moore NC, Girdlestone J, Anderson G, Owen JJ, Jenkinson EJ (1995) Stimulation of thymocytes before and after positive selection results in the induction of different NF-kappa B/Rel protein complexes. J Immunol 155: 4653-4660.
  43. Burkly L, Hession C, Ogata L, Reilly C, Marconi LA, et al. (1995) Expression of relB is required for the development of thymic medulla and dendritic cells. Nature 373: 531-536.
  44. Gerondakis S, Grumont R, Gugasyan R, Wong L, Isomura I, et al. (2006) Unravelling the complexities of the NF-kappaB signalling pathway using mouse knockout and transgenic models. Oncogene 25: 6781-6799.
  45. Boothby MR, Mora AL, Scherer DC, Brockman JA, Ballard DW (1997) Perturbation of the T lymphocyte lineage in transgenic mice expressing a constitutive repressor of nuclear factor (NF)-kappaB. J Exp Med 185: 1897-1907.
  46. Mora AL, Stanley S, Armistead W, Chan AC, Boothby M (2001) Inefficient ZAP-70 phosphorylation and decreased thymic selection in vivo result from inhibition of NF-kappaB/Rel. J Immunol 167: 5628-5635.
  47. Hettmann T, Leiden JM (2000) NF-kappa B is required for the positive selection of CD8+ thymocytes. J Immunol 165: 5004-5010.
  48. Hettmann T, DiDonato J, Karin M, Leiden JM (1999) An essential role for nuclear factor kappaB in promoting double positive thymocyte apoptosis. J Exp Med 189: 145-158.
  49. Ren H, Schmalstieg A, van Oers NS, Gaynor RB (2002) I-kappa B kinases alpha and beta have distinct roles in regulating murine T cell function. J Immunol 168: 3721-3731.
  50. Schmidt-Supprian M, Courtois G, Tian J, Coyle AJ, Israël A, et al. (2003) Mature T cells depend on signaling through the IKK complex. Immunity 19: 377-389.
  51. Fiorini E, Schmitz I, Marissen WE, Osborn SL, Touma M, et al. (2002) Peptide-induced negative selection of thymocytes activates transcription of an NF-kappa B inhibitor. Mol Cell 9: 637-648.
  52. Jimi E, Strickland I, Voll RE, Long M, Ghosh S (2008) Differential role of the transcription factor NF-kappaB in selection and survival of CD4+ and CD8+ thymocytes. Immunity 29: 523-537.
  53. Isomura I, Palmer S, Grumont RJ, Bunting K, Hoyne G, et al. (2009) c-Rel is required for the development of thymic Foxp3+ CD4 regulatory T cells. J Exp Med 206: 3001-3014.
  54. Deenick EK, Elford AR, Pellegrini M, Hall H, Mak TW, et al. (2010) c-Rel but not NF-kappaB1 is important for T regulatory cell development. Eur J Immunol 40: 677-681.
  55. Sivakumar V, Hammond KJ, Howells N, Pfeffer K, Weih F (2003) Differential requirement for Rel/nuclear factor kappa B family members in natural killer T cell development. J Exp Med 197: 1613-1621.
  56. Stankovic S, Gugasyan R, Kyparissoudis K, Grumont R, Banerjee A, et al. (2011) Distinct roles in NKT cell maturation and function for the different transcription factors in the classical NF-κB pathway. Immunol Cell Biol 89: 294-303.
  57. Gugasyan R, Horat E, Kinkel SA, Ross F, Grigoriadis G, et al. (2011) The NF-κB1 transcription factor prevents the intrathymic development of CD8 T cells with memory properties. EMBO J 31: 692-706.
  58. Elewaut D, Shaikh RB, Hammond KJ, De Winter H, Leishman AJ, et al. (2003) NIK-dependent RelB activation defines a unique signaling pathway for the development of V alpha 14i NKT cells. J Exp Med 197: 1623-1633.
  59. Ruan Q, Kameswaran V, Tone Y, Li L, Liou HC, et al. (2009) Development of Foxp3(+) regulatory t cells is driven by the c-Rel enhanceosome. Immunity 31: 932-940.
  60. Long M, Park SG, Strickland I, Hayden MS, Ghosh S (2009) Nuclear factor-kappaB modulates regulatory T cell development by directly regulating expression of Foxp3 transcription factor. Immunity 31: 921-931.
  61. Zheng Y, Josefowicz S, Chaudhry A, Peng XP, Forbush K, et al. (2010) Role of conserved non-coding DNA elements in the Foxp3 gene in regulatory T-cell fate. Nature 463: 808-812.
  62. Grigoriadis G, Vasanthakumar A, Banerjee A, Grumont R, Overall S, et al. (2011) c-Rel controls multiple discrete steps in the thymic development of Foxp3+ CD4 regulatory T cells. PLoS One 6: e26851.
  63. Lu LF, Gondek DC, Scott ZA, Noelle RJ (2005) NF kappa B-inducing kinase deficiency results in the development of a subset of regulatory T cells, which shows a hyperproliferative activity upon glucocorticoid-induced TNF receptor family-related gene stimulation. J Immunol 175: 1651-1657.
  64. Murray SE, Polesso F, Rowe AM, Basak S, Koguchi Y, et al. (2011) NF-κB-inducing kinase plays an essential T cell–intrinsic role in graft-versus-host disease and lethal autoimmunity in mice. J Clin Invest 121: 4775-4786.
  65. Fukuyama T, Kasper LH, Boussouar F, Jeevan T, van Deursen J, et al. (2009) Histone acetyltransferase CBP is vital to demarcate conventional and innate CD8+ T-cell development. Mol Cell Biol 29: 3894-3904.
  66. Weinreich MA, Odumade OA, Jameson SC, Hogquist KA (2010) T cells expressing the transcription factor PLZF regulate the development of memory-like CD8+ T cells. Nat Immunol 11: 709-716.
  67. Atherly LO, Lucas JA, Felices M, Yin CC, Reiner SL, et al. (2006) The Tec family tyrosine kinases Itk and Rlk regulate the development of conventional CD8+ T cells. Immunity 25: 79-91.
  68. Sharma A, Chen Q, Nguyen T, Yu Q, Sen JM (2012) T cell factor-1 and ß-catenin control the development of memory-like CD8 thymocytes. J Immunol 188: 3859-3868.
  69. Verykokakis M, Boos MD, Bendelac A, Kee BL (2010) SAP protein-dependent natural killer T-like cells regulate the development of CD8(+) T cells with innate lymphocyte characteristics. Immunity 33: 203-215.
  70. Pearce EL, Mullen AC, Martins GA, Krawczyk CM, Hutchins AS, et al. (2003) Control of effector CD8+ T cell function by the transcription factor Eomesodermin. Science 302: 1041-1043.
  71. Prince AL, Yin CC, Enos ME, Felices M, Berg LJ (2009) The Tec kinases Itk and Rlk regulate conventional versus innate T-cell development. Immunol Rev 228: 115-131.
  72. Urdahl KB, Sun JC, Bevan MJ (2002) Positive selection of MHC class Ib-restricted CD8(+) T cells on hematopoietic cells. Nat Immunol 3: 772-779.
  73. Kurepa Z, Su J, Forman J (2003) Memory phenotype of CD8+ T cells in MHC class Ia-deficient mice. J Immunol 170: 5414-5420.
  74. Lai D, Zhu J, Wang T, Hu-Li J, Terabe M, et al. (2011) KLF13 sustains thymic memory-like CD8(+) T cells in BALB/c mice by regulating IL-4-generating invariant natural killer T cells. J Exp Med 208: 1093-1103.
  75. Guida JD, Fejer G, Pirofski LA, Brosnan CF, Horwitz MS (1995) Mouse adenovirus type 1 causes a fatal hemorrhagic encephalomyelitis in adult C57BL/6 but not BALB/c mice. J Virol 69: 7674-7681.
  76. Niewiesk S, Brinckmann U, Bankamp B, Sirak S, Liebert UG, et al. (1993) Susceptibility to measles virus-induced encephalitis in mice correlates with impaired antigen presentation to cytotoxic T lymphocytes. J Virol 67: 75-81.
  77. Gattinoni L, Lugli E, Ji Y, Pos Z, Paulos CM, et al. (2011) A human memory T cell subset with stem cell-like properties. Nat Med 17: 1290-1297.
  78. Linka RM, Risse SL, Bienemann K, Werner M, Linka Y, et al. (2012) Loss-of-function mutations within the IL-2 inducible kinase ITK in patients with EBV-associated lymphoproliferative diseases. Leukemia 26: 963-971.
  79. Huck K, Feyen O, Niehues T, Rüschendorf F, Hübner N, et al. (2009) Girls homozygous for an IL-2-inducible T cell kinase mutation that leads to protein deficiency develop fatal EBV-associated lymphoproliferation. J Clin Invest 119: 1350-1358.
  80. Turtle CJ, Swanson HM, Fujii N, Estey EH, Riddell SR (2009) A distinct subset of self-renewing human memory CD8+ T cells survives cytotoxic chemotherapy. Immunity 31: 834-844.
  81. Kang SM, Tran AC, Grilli M, Lenardo MJ (1992) NF-kappa B subunit regulation in nontransformed CD4+ T lymphocytes. Science 256: 1452-1456.
  82. Hilliard BA, Mason N, Xu L, Sun J, Lamhamedi-Cherradi SE, et al. (2002) Critical roles of c-Rel in autoimmune inflammation and helper T cell differentiation. J Clin Invest 110: 843-850.
  83. Corn RA, Aronica MA, Zhang F, Tong Y, Stanley SA, et al. (2003) T cell-intrinsic requirement for NF-kappa B induction in postdifferentiation IFN-gamma production and clonal expansion in a Th1 response. J Immunol 171: 1816-1824.
  84. Schmidt-Supprian M, Tian J, Grant EP, Pasparakis M, Maehr R, et al. (2004) Differential dependence of CD4+CD25+ regulatory and natural killer-like T cells on signals leading to NF-kappaB activation. Proc Natl Acad Sci U S A 101: 4566-4571.
  85. Grumont R, Lock P, Mollinari M, Shannon FM, Moore A, et al. (2004) The mitogen-induced increase in T cell size involves PKC and NFAT activation of Rel/NF-kappaB-dependent c-myc expression. Immunity 21: 19-30.
  86. Mason NJ, Liou HC, Hunter CA (2004) T cell-intrinsic expression of c-Rel regulates Th1 cell responses essential for resistance to Toxoplasma gondii. J Immunol 172: 3704-3711.
  87. Caamaño J, Alexander J, Craig L, Bravo R, Hunter CA (1999) The NF-kappa B family member RelB is required for innate and adaptive immunity to Toxoplasma gondii. J Immunol 163: 4453-4461.
  88. Das J, Chen CH, Yang L, Cohn L, Ray P, et al. (2001) A critical role for NF-kappa B in GATA3 expression and TH2 differentiation in allergic airway inflammation. Nat Immunol 2: 45-50.
  89. Grumont R, Hochrein H, O'Keeffe M, Gugasyan R, White C, et al. (2001) c-Rel regulates interleukin 12 p70 expression in CD8(+) dendritic cells by specifically inducing p35 gene transcription. J Exp Med 194: 1021-1032.
  90. Wang J, Wang X, Hussain S, Zheng Y, Sanjabi S, et al. (2007) Distinct roles of different NF-kappa B subunits in regulating inflammatory and T cell stimulatory gene expression in dendritic cells. J Immunol 178: 6777-6788.
  91. Molitor JA, Walker WH, Doerre S, Ballard DW, Greene WC (1990) NF-kappa B: a family of inducible and differentially expressed enhancer-binding proteins in human T cells. Proc Natl Acad Sci U S A 87: 10028-10032.
  92. Grumont RJ, Gerondakis S (1994) The subunit composition of NF-kappa B complexes changes during B-cell development. Cell Growth Differ 5: 1321-1331.
  93. Köntgen F, Grumont RJ, Strasser A, Metcalf D, Li R, et al. (1995) Mice lacking the c-rel proto-oncogene exhibit defects in lymphocyte proliferation, humoral immunity, and interleukin-2 expression. Genes Dev 9: 1965-1977.
  94. Rao S, Gerondakis S, Woltring D, Shannon MF (2003) c-Rel is required for chromatin remodeling across the IL-2 gene promoter. J Immunol 170: 3724-3731.
  95. Schmidt-Supprian M, Tian J, Ji H, Terhorst C, Bhan AK, et al. (2004) I kappa B kinase 2 deficiency in T cells leads to defects in priming, B cell help, germinal center reactions, and homeostatic expansion. J Immunol 173: 1612-1619.
  96. Sriskantharajah S, Belich MP, Papoutsopoulou S, Janzen J, Tybulewicz V, et al. (2009) Proteolysis of NF-kappaB1 p105 is essential for T cell antigen receptor-induced proliferation. Nat Immunol 10: 38-47.
  97. Zheng Y, Vig M, Lyons J, Van Parijs L, Beg AA (2003) Combined deficiency of p50 and cRel in CD4+ T cells reveals an essential requirement for nuclear factor kappaB in regulating mature T cell survival and in vivo function. J Exp Med 197: 861-874.
  98. Aronica MA, Mora AL, Mitchell DB, Finn PW, Johnson JE, et al. (1999) Preferential role for NF-kappa B/Rel signaling in the type 1 but not type 2 T cell-dependent immune response in vivo. J Immunol 163: 5116-5124.
  99. Corn RA, Hunter C, Liou HC, Siebenlist U, Boothby MR (2005) Opposing roles for RelB and Bcl-3 in regulation of T-box expressed in T cells, GATA-3, and Th effector differentiation. J Immunol 175: 2102-2110.
  100. Harrington LE, Hatton RD, Mangan PR, Turner H, Murphy TL, et al. (2005) Interleukin 17-producing CD4+ effector T cells develop via a lineage distinct from the T helper type 1 and 2 lineages. Nat Immunol 6: 1123-1132.
  101. Waite JC, Skokos D (2012) Th17 response and inflammatory autoimmune diseases. Int J Inflam 2012: 819467.
  102. Chen G, Hardy K, Pagler E, Ma L, Lee S, et al. (2011) The NF-κB transcription factor c-Rel is required for Th17 effector cell development in experimental autoimmune encephalomyelitis. J Immunol 187: 4483-4491.
  103. Ruan Q, Kameswaran V, Zhang Y, Zheng S, Sun J, et al. (2011) The Th17 immune response is controlled by the Rel-RORγ-RORγ T transcriptional axis. J Exp Med 208: 2321-2333.
  104. Jin W, Zhou XF, Yu J, Cheng X, Sun SC (2009) Regulation of Th17 cell differentiation and EAE induction by MAP3K NIK. Blood 113: 6603-6610.
  105. Hofmann J, Mair F, Greter M, Schmidt-Supprian M, Becher B (2011) NIK signaling in dendritic cells but not in T cells is required for the development of effector T cells and cell-mediated immune responses. J Exp Med 208: 1917-1929.
  106. Crotty S (2011) Follicular helper CD4 T cells (TFH). Annu Rev Immunol 29: 621-663.
  107. Chen G, Hardy K, Bunting K, Daley S, Ma L, et al. (2010) Regulation of the IL-21 gene by the NF-κB transcription factor c-Rel. J Immunol 185: 2350-2359.
  108. Vogelzang A, McGuire HM, Yu D, Sprent J, Mackay CR, et al. (2008) A fundamental role for interleukin-21 in the generation of T follicular helper cells. Immunity 29: 127-137.
  109. Nurieva RI, Chung Y, Hwang D, Yang XO, Kang HS, et al. (2008) Generation of T follicular helper cells is mediated by interleukin-21 but independent of T helper 1, 2, or 17 cell lineages. Immunity 29: 138-149.
  110. Hu H, Wu X, Jin W, Chang M, Cheng X, et al. (2011) Noncanonical NF-kappaB regulates inducible costimulator (ICOS) ligand expression and T follicular helper cell development. Proc Natl Acad Sci U S A 108: 12827-12832.
  111. Li Z, Wang X, Yu RY, Ding BB, Yu JJ, et al. (2005) BCL-6 negatively regulates expression of the NF-kappaB1 p105/p50 subunit. J Immunol 174: 205-214.
  112. Kieran M, Blank V, Logeat F, Vandekerckhove J, Lottspeich F, et al. (1990) The DNA binding subunit of NF-kappa B is identical to factor KBF1 and homologous to the rel oncogene product. Cell 62: 1007-1018.
  113. Ghosh S, Gifford AM, Riviere LR, Tempst P, Nolan GP, et al. (1990) Cloning of the p50 DNA binding subunit of NF-kappa B: homology to rel and dorsal. Cell 62: 1019-1029.
  114. Bassères DS, Baldwin AS (2006) Nuclear factor-kappaB and inhibitor of kappaB kinase pathways in oncogenic initiation and progression. Oncogene 25: 6817-6830.
  115. Sethi G, Sung B, Aggarwal BB (2008) Nuclear factor-kappaB activation: from bench to bedside. Exp Biol Med (Maywood) 233: 21-31.
  116. Karin M (2009) NF-kappaB as a critical link between inflammation and cancer. Cold Spring Harb Perspect Biol 1: a000141.
  117. Rayet B, Gélinas C (1999) Aberrant rel/nfkb genes and activity in human cancer. Oncogene 18: 6938-6947.
  118. Courtois G, Gilmore TD (2006) Mutations in the NF-kappaB signaling pathway: implications for human disease. Oncogene 25: 6831-6843.
  119. Barth TF, Martin-Subero JI, Joos S, Menz CK, Hasel C, et al. (2003) Gains of 2p involving the REL locus correlate with nuclear c-Rel protein accumulation in neoplastic cells of classical Hodgkin lymphoma. Blood 101: 3681-3686.
  120. Joos S, Granzow M, Holtgreve-Grez H, Siebert R, Harder L, et al. (2003) Hodgkin's lymphoma cell lines are characterized by frequent aberrations on chromosomes 2p and 9p including REL and JAK2. Int J Cancer 103: 489-495.
  121. Neri A, Fracchiolla NS, Migliazza A, Trecca D, Lombardi L (1996) The involvement of the candidate proto-oncogene NFKB2/lyt-10 in lymphoid malignancies. Leuk Lymphoma 23: 43-48.
  122. Izban KF, Ergin M, Huang Q, Qin JZ, Martinez RL, et al. (2001) Characterization of NF-kappaB expression in Hodgkin's disease: inhibition of constitutively expressed NF-kappaB results in spontaneous caspase-independent apoptosis in Hodgkin and Reed-Sternberg cells. Mod Pathol 14: 297-310.
  123. Kordes U, Krappmann D, Heissmeyer V, Ludwig WD, Scheidereit C (2000) Transcription factor NF-kappaB is constitutively activated in acute lymphoblastic leukemia cells. Leukemia 14: 399-402.
  124. Davis RE, Brown KD, Siebenlist U, Staudt LM (2001) Constitutive nuclear factor kappaB activity is required for survival of activated B cell-like diffuse large B cell lymphoma cells. J Exp Med 194: 1861-1874.
  125. Perkins ND (2004) NF-kappaB: tumor promoter or suppressor? Trends Cell Biol 14: 64-69.
  126. Bargou RC, Emmerich F, Krappmann D, Bommert K, Mapara MY, et al. (1997) Constitutive nuclear factor-kappaB-RelA activation is required for proliferation and survival of Hodgkin's disease tumor cells. J Clin Invest 100: 2961-2969.
  127. Mathas S, Jöhrens K, Joos S, Lietz A, Hummel F, et al. (2005) Elevated NF-kappaB p50 complex formation and Bcl-3 expression in classical Hodgkin, anaplastic large-cell, and other peripheral T-cell lymphomas. Blood 106: 4287-4293.
  128. Ni H, Ergin M, Huang Q, Qin JZ, Amin HM, et al. (2001) Analysis of expression of nuclear factor kappa B (NF-kappa B) in multiple myeloma: downregulation of NF-kappa B induces apoptosis. Br J Haematol 115: 279-286.
  129. Sun XF, Zhang H (2007) NFKB and NFKBI polymorphisms in relation to susceptibility of tumour and other diseases. Histol Histopathol 22: 1387-1398.
  130. Zou YF, Wang F, Feng XL, Tao JH, Zhu JM, et al. (2011) Association of NFKB1 -94ins/delATTG promoter polymorphism with susceptibility to autoimmune and inflammatory diseases: a meta-analysis. Tissue Antigens 77: 9-17.
  131. Anest V, Hanson JL, Cogswell PC, Steinbrecher KA, Strahl BD, et al. (2003) A nucleosomal function for IkappaB kinase-alpha in NF-kappaB-dependent gene expression. Nature 423: 659-663.
  132. Sil AK, Maeda S, Sano Y, Roop DR, Karin M (2004) IkappaB kinase-alpha acts in the epidermis to control skeletal and craniofacial morphogenesis. Nature 428: 660-664.
  133. Luo JL, Tan W, Ricono JM, Korchynskyi O, Zhang M, et al. (2007) Nuclear cytokine-activated IKKalpha controls prostate cancer metastasis by repressing Maspin. Nature 446: 690-694.
  134. Li L, Ruan Q, Hilliard B, Devirgiliis J, Karin M, et al. (2011) Transcriptional regulation of the Th17 immune response by IKK(alpha). J Exp Med 208: 787-796.
  135. Osipo C, Golde TE, Osborne BA, Miele LA (2008) Off the beaten pathway: the complex cross talk between Notch and NF-kappaB. Lab Invest 88: 11-17.
  136. Song LL, Peng Y, Yun J, Rizzo P, Chaturvedi V, et al. (2008) Notch-1 associates with IKKalpha and regulates IKK activity in cervical cancer cells. Oncogene 27: 5833-5844.
  137. Shin HM, Minter LM, Cho OH, Gottipati S, Fauq AH, et al. (2006) Notch1 augments NF-kappaB activity by facilitating its nuclear retention. EMBO J 25: 129-138.
  138. Riz I, Zweier-Renn LA, Toma I, Hawley TS, Hawley RG (2011) Apoptotic role of IKK in T-ALL therapeutic response. Mol Cancer Res 9: 979-984.
  139. Liu M, Lee DF, Chen CT, Yen CJ, Li LY, et al. (2012) IKKa activation of NOTCH links tumorigenesis via FOXA2 suppression. Mol Cell 45: 171-184.
  140. Ravi R, Mookerjee B, van Hensbergen Y, Bedi GC, Giordano A, et al. (1998) p53-mediated repression of nuclear factor-kappaB RelA via the transcriptional integrator p300. Cancer Res 58: 4531-4536.
  141. Huang WC, Ju TK, Hung MC, Chen CC (2007) Phosphorylation of CBP by IKKalpha promotes cell growth by switching the binding preference of CBP from p53 to NF-kappaB. Mol Cell 26: 75-87.
  142. Schumm K, Rocha S, Caamano J, Perkins ND (2006) Regulation of p53 tumour suppressor target gene expression by the p52 NF-kappaB subunit. EMBO J 25: 4820-4832.
  143. Nesic D, Grumont R, Gerondakis S (2008) The nuclear factor-kappaB and p53 pathways function independently in primary cells and transformed fibroblasts responding to genotoxic damage. Mol Cancer Res 6: 1193-1203.
  144. Salmeron A, Ahmad TB, Carlile GW, Pappin D, Narsimhan RP, et al. (1996) Activation of MEK-1 and SEK-1 by Tpl-2 proto-oncoprotein, a novel MAP kinase kinase kinase. EMBO J 15: 817-826.
  145. Waterfield MR, Zhang M, Norman LP, Sun SC (2003) NF-kappaB1/p105 regulates lipopolysaccharide-stimulated MAP kinase signaling by governing the stability and function of the Tpl2 kinase. Mol Cell 11: 685-694.
  146. Beinke S, Deka J, Lang V, Belich MP, Walker PA, et al. (2003) NF-kappaB1 p105 negatively regulates TPL-2 MEK kinase activity. Mol Cell Biol 23: 4739-4752.
  147. Lee S, Andrieu C, Saltel F, Destaing O, Auclair J, et al. (2004) IkappaB kinase beta phosphorylates Dok1 serines in response to TNF, IL-1, or gamma radiation. Proc Natl Acad Sci U S A 101: 17416-17421.
  148. Tsatsanis C, Patriotis C, Bear SE, Tsichlis PN (1998) The Tpl-2 protooncoprotein activates the nuclear factor of activated T cells and induces interleukin 2 expression in T cell lines. Proc Natl Acad Sci U S A 95: 3827-3832.
  149. Belich MP, Salmerón A, Johnston LH, Ley SC (1999) TPL-2 kinase regulates the proteolysis of the NF-kappaB-inhibitory protein NF-kappaB1 p105. Nature 397: 363-368.
  150. Sánchez-Valdepeñas C, Punzón C, San-Antonio B, Martin AG, Fresno M (2007) Differential regulation of p65 and c-Rel NF-kappaB transactivating activity by Cot, protein kinase C zeta and NIK protein kinases in CD3/CD28 activated T cells. Cell Signal 19: 528-537.
  151. Dumitru CD, Ceci JD, Tsatsanis C, Kontoyiannis D, Stamatakis K, et al. (2000) TNF-alpha induction by LPS is regulated posttranscriptionally via a Tpl2/ERK-dependent pathway. Cell 103: 1071-1083.
  152. Fischer AM, Katayama CD, Pagès G, Pouysségur J, Hedrick SM (2005) The role of erk1 and erk2 in multiple stages of T cell development. Immunity 23: 431-443.
  153. O'Shea CC, Crompton T, Rosewell IR, Hayday AC, Owen MJ (1996) Raf regulates positive selection. Eur J Immunol 26: 2350-2355.
  154. Watford WT, Hissong BD, Durant LR, Yamane H, Muul LM, et al. (2008) Tpl2 kinase regulates T cell interferon-gamma production and host resistance to Toxoplasma gondii. J Exp Med 205: 2803-2812.
  155. Watford WT, Wang CC, Tsatsanis C, Mielke LA, Eliopoulos AG, et al. (2010) Ablation of tumor progression locus 2 promotes a type 2 Th cell response in Ovalbumin-immunized mice. J Immunol 184: 105-113.
  156. Sugimoto K, Ohata M, Miyoshi J, Ishizaki H, Tsuboi N, et al. (2004) A serine/threonine kinase, Cot/Tpl2, modulates bacterial DNA-induced IL-12 production and Th cell differentiation. J Clin Invest 114: 857-866.
  157. Miyoshi J, Higashi T, Mukai H, Ohuchi T, Kakunaga T (1991) Structure and transforming potential of the human cot oncogene encoding a putative protein kinase. Mol Cell Biol 11: 4088-4096.
  158. Patriotis C, Makris A, Bear SE, Tsichlis PN (1993) Tumor progression locus 2 (Tpl-2) encodes a protein kinase involved in the progression of rodent T-cell lymphomas and in T-cell activation. Proc Natl Acad Sci U S A 90: 2251-2255.
  159. Ceci JD, Patriotis CP, Tsatsanis C, Makris AM, Kovatch R, et al. (1997) Tpl-2 is an oncogenic kinase that is activated by carboxy-terminal truncation. Genes Dev 11: 688-700.
  160. Tsatsanis C, Vaporidi K, Zacharioudaki V, Androulidaki A, Sykulev Y, et al. (2008) Tpl2 and ERK transduce antiproliferative T cell receptor signals and inhibit transformation of chronically stimulated T cells. Proc Natl Acad Sci U S A 105: 2987-2992.
  161. Christoforidou AV, Papadaki HA, Margioris AN, Eliopoulos GD, Tsatsanis C (2004) Expression of the Tpl2/Cot oncogene in human T-cell neoplasias. Mol Cancer 3: 34.
  162. Vougioukalaki M, Kanellis DC, Gkouskou K, Eliopoulos AG (2011) Tpl2 kinase signal transduction in inflammation and cancer. Cancer Lett 304: 80-89.
  163. Hayden MS, Ghosh S (2012) NF-κB, the first quarter-century: remarkable progress and outstanding questions. Genes Dev 26: 203-234.
  164. Hu J, Sahu N, Walsh E, August A (2007) Memory phenotype CD8+ T cells with innate function selectively develop in the absence of active Itk. Eur J Immunol 37: 2892-2899.
  165. Schaeffer EM, Broussard C, Debnath J, Anderson S, McVicar DW, et al. (2000) Tec family kinases modulate thresholds for thymocyte development and selection. J Exp Med 192: 987-1000.
  166. Lucas JA, Atherly LO, Berg LJ (2002) The absence of Itk inhibits positive selection without changing lineage commitment. J Immunol 168: 6142-6151.
  167. Judge AD, Zhang X, Fujii H, Surh CD, Sprent J (2002) Interleukin 15 controls both proliferation and survival of a subset of memory-phenotype CD8(+) T cells. J Exp Med 196: 935-946.
  168. Dubois S, Waldmann TA, Müller JR (2006) ITK and IL-15 support two distinct subsets of CD8+ T cells. Proc Natl Acad Sci U S A 103: 12075-12080.
  169. Broussard C, Fleischacker C, Horai R, Chetana M, Venegas AM, et al. (2006) Altered development of CD8+ T cell lineages in mice deficient for the Tec kinases Itk and Rlk. Immunity 25: 93-104.
  170. Horai R, Mueller KL, Handon RA, Cannons JL, Anderson SM, et al. (2007) Requirements for selection of conventional and innate T lymphocyte lineages. Immunity 27: 775-785.
  171. Li H, Dai M, Zhuang Y (2004) A T cell intrinsic role of Id3 in a mouse model for primary Sjogren's syndrome. Immunity 21: 551-560.
  172. Guo Z, Li H, Han M, Xu T, Wu X, et al. (2011) Modeling Sjögren's syndrome with Id3 conditional knockout mice. Immunol Lett 135: 34-42.
Citation: de Valle E, Lie LK, Berzins SP, Gugasyan R (2012) The Role of NFκB in T-lymphocyte Development and Function. J Clin Cell Immunol S12:009

Copyright: © 2012 de Valle E, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Top