Journal of Hematology & Thromboembolic Diseases
Open Access

Prevalence of Anemia and Associated Risk Factors among Type 2 Diabetic Mellitus Patients Attending Ambo Town Government Hospitals in West Shewa, Ethiopia

Jafero Oljira, Fekadu Urgessa^* and Mintewab Hussein ^*Correspondence: Fekadu Urgessa, Department of Medical Laboratory Sciences, College of Health Science, Addis Ababa University, Addis Ababa, Ethiopia, Tel: +251934442555, Email:

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Abbreviations

ACE: Angiotension Converting Enzyme; AOR: Adjusted Odds Ratio; BMI: Body Mass Index; BP: Blood Pressure; CKD: Chronic Kidney Disease; EDTA; Ethylenediamine tetra acetic acid; DM: Diabetes Mellitus; eGFR: Estimated Glomerular Filtration Rate; Hgb: Hemoglobin; IL: Interleukin; K/DOQI: Kidney Disease Outcomes Quality Initiatives; MDRD: Modification of Diet in Renal Disease; SOP: Standard Operating Procedure; SST: Serum Separation Tube; T2DM: Type two Diabetes Mellitus; WHO: World health organization.

Introduction

Anemia is the most common blood disorder and a common finding in patients with diabetes. It is also considered a key indicator of chronic kidney disease and an important cardiovascular risk factor [1]. Patients with diabetes mellitus are also twice as likely to have anemia as those with renal impairment from other causes. Furthermore, declining hemoglobin levels may be observed before changes in renal function [2]. It results from diminished erythropoietin production and to a lesser degree due to the increased excretion of erythropoietin in urine [3]. Different findings show anemia in T2DM patients is a strong and independent indicator of increased risk for diabetes related macrovascular and microvascular complications. It causes early occurrence and rapid progression of complications like diabetic nephropathy, diabetic retinopathy, diabetic neuropathy, end-stage renal diseases, ischemic heart disease, and non-healing diabetic foot ulcer [4].

The incidence and prevalence of anemia in patients with diabetes is typically associated with erythropoietin deficiency due to concomitant renal disease [5,6]. Furthermore, anemia is a common complication of diabetes mellitus, and the risk of anemia in diabetic patients is estimated to be two to three times higher than that of patients without diabetes [7]. The risk of developing anemia in patients with diabetes and renal disease is significantly greater, and is often more severe, and occurs earlier than in non-diabetic patients with renal diseases [8,9]. Recent studies have demonstrated the unfavorable influence of anemia on the progression of renal disease, cost of managing the disease, quality of life and cardiovascular disease, and all-cause mortality in diabetic patients who also have renal disease and the combination of anemia and kidney disease in diabetics identifies a group with adverse outcomes [10-12]. However, there are other factors that could contribute to the development of anemia in diabetes, including chronic inflammation, oxidative stress, autonomic neuropathy, nutritional deficiencies (iron, folate, and vitamin B₁₂), autoimmune diseases, drugs, and advanced glycation [13].

Anemia is a global public health problem and it occurs at all stages of the life cycle and affects nearly two billion (27%) people worldwide [14]. Globally, the prevalence of concurrent anemia and diabetes mellitus (both type1 and type2) ranges from 14% to 45% in various ethnic populations worldwide. The magnitude of anemia among T2DM patients varies among studies and regions, ranging from 7.7% in the United States of America (USA) to 67% in India [15-20]. The cause of anemia in diabetes is multi-factorial including nutritional deficiencies, inflammation, concomitant autoimmune diseases and advanced age. Further, lower Body Mass Index (BMI), longer duration of diabetes, peripheral vascular disease, specific medications, and hormonal changes could be mentioned in addition to kidney disease. Various studies revealed the development of anemia in T2DM patients is significantly associated with sex, age, marital status, educational status, BMI, hypertension, hematological diseases, glycemic control, gastrointestinal disorders, and chronic kidney diseases. The duration of diabetes and microvascular complications of diabetes such as diabetic nephropathy, neuropathy, and retinopathy have all found to be significantly associated with anemia in T2DM patients. In spite of many types of research were done on the prevalence of anemia among T2DM, anemia in T2DM remains unrecognized and untreated in 25% of the diabetic patients because both share similar symptoms such as lethargy, pale skin, chest pain, irritability, numbness/coldness in the hands and feet, tachycardia, shortness of breath and headache (Figure 1).

Figure 1: Conceptual Framework developed after reviewing work of different works of literature

Our country, Ethiopia is one of the developing countries where both anemia and diabetes mellitus are major public health issues with an increased incidence of DM it becomes mandatory to be aware of such co-morbidities at the earliest. Therefore, this study will aim to incorporate anemia screening in the guideline on the management of diabetes and to determine the prevalence of anemia and its associated risk factors among patients with type 2 diabetic mellitus patients attending Ambo town government hospitals, West Shewa, Ethiopia.

Materials and Methods

Study area, period and design

The study was conducted at Ambo town in government hospitals, west shewa, Oromia, Ethiopia. Ambo town is located 115 km from the capital city of Ethiopia, Addis Ababa. A hospital-based cross-sectional study was conducted at Ambo town in government hospitals from September 2020-October 2020 G.C. The source population includes all type two diabetic patients seeking healthcare services at Ambo town government hospitals. The study population includes all type two diabetic patients seeking healthcare services at Ambo town government hospitals for follow-up from September through October 2020 G.C.

Inclusion and exclusion criteria

Inclusion criteria: Patients with diagnosed type 2 diabetes, irrespective of gender and aged 18 years or above were included.

Exclusion criteria: Participants, who are seriously ill, and unable to stand and sit without assistance or support, were excluded from the study because it is difficult to get an appropriate and accurate measurement from these patients.

Study variables

Dependent variables: Prevalence of anemia.

Independent variables

Socio-demographic characteristics: Age, sex, marital status, occupational status, educational status, and residence.

Lifestyle characteristics: Cigarette smoking, alcohol consumption, physical activity, Nutritional status (Body Mass Index or BMI).

Clinical conditions: Duration of diabetes (in years), medications taken for diabetes, medications taken for diseases other than diabetes, types of diseases for which other medications taken,neuropathy, retinopathy, nephropathy, history of hypertension, HIV status, history of blood loss, glycemic control, systolic blood pressure (mmHg), diastolic blood pressure (mmHg), serum creatinine and urine albumin.

Sample size calculation: By using single proportion sample size determination, the sample size was estimated assuming a prevalence of anemia of 34.8% based on a previous study in people with diabetes in Harari Region Eastern Ethiopia, a precision of 5% and a z value of 1.96. By adding 10% for nonrespondent a total of 383 participants were required. A simple random sampling procedure was used to select the study participants. Patients with missing the clinical data were excluded, leaving 356 patients for the final analysis.

Data collection and laboratory measurement: Data were collected by using a semi-structured questionnaire. The collected information includes socio-demographic characteristics, clinical characteristics, anthropometric measurements, and laboratory analysis. Sociodemographic data and clinical characteristics like duration of DM were collected using an interview guide; whereas the presence of diabetes related complications like; retinopathy, neuropathy, nephropathy, and other complication; history of hypertension and current diabetic medications were collected from reviewing of patient’s medical records. The duration of diabetes is categorized as less than five years and greater than or equal to five years. Four consecutive fasting blood glucose measurements, including measurement at the time of the data collection period, were also recorded from the patient’s medical records for calculating the mean blood glucose level. Anthropometric measurements such as weight (kg) and height (m) were measured according to WHO recommendations. The Body Mass Index (BMI) was computed as weight in kilograms divided by the square of the height in meters (kg/m²). The BMI of the participants were classified as: Underweight less than 18.5 kg/m², normal (18.5 kg/m²-24.9 kg/m²), overweight (25 kg/m2-29.9 kg/m²), and obese (≥ 30 kg/m²). Blood Pressure (BP) was measured by using a manual sphygmomanometer after 10 mins of rest in a sitting position hypertension was defined as Systolic Blood Pressure (SBP) ≥140 mmHg and/or Diastolic Blood Pressure (DBP) ≥90 mmHg. For laboratory data, from each participant, five mL of venous blood was collected under aseptic conditions by venous puncture from the vein using a disposable syringe for hemoglobin determination, and serum creatinine analysis. Hemoglobin (Hgb) values were calculated by using a hematology analyzer, mission hemoglobin meter. Serum creatinine was analyzed by BS-200 analyzer chemistry analyzer computed and as mg/dl. Serum creatinine values were considered abnormal if values of serum creatinine analysis were >1.2 mg/dl. Good glycemic control: an average of four consecutive fasting blood glucose measurements was ≤ 130 mg/dl and poor glycemic control: An average of four consecutive fasting blood glucose measurements was >130 mg/d. Urine samples were collected from the participating patients to detect the presence of albumin which indicates abnormal and the absence of albumin indicates that normal by using dipsticks [2].

Data quality assurance

The principal investigator supervised the data collection process. The training was given to the data collectors. The completeness of data collected was checked every day by the principal The reliability of the study findings is guaranteed by implementing Quality Control (QC) measures throughout the investigator.whole process of the laboratory works. All materials, equipment, and procedures were adequately controlled.

Pre-analytical: In this phase, the principal investigator checked the sample quality and its accuracy by checking the patient request with full information and proper labeling, handling, and transportation of the sample. Based on sample acceptance and rejection criteria the sample was evaluated for clot, hemolysis, and lipemic. Any sample with that problem was rejected and also patient request that is not properly filled with necessary patient information and miss-match of sample in identification was rejected.

Analytical phase: In this phase, the patient specimen was prepared for testing and ends when the test result is interpreted and verified. Doing this the specimen was rechecked for its correct labeling and identifications again by the personnel who analyze the sample. After checking the sample accuracy the personnel checked the machine and by doing daily, weekly, or as needed maintenance. After doing the maintenance the personnel processed the daily QC.

Post-analytical phase: Post-analytical quality assurance was insured by using appropriate result formats and registrations.

Data analysis and interpretation

The data was entered into “Epi info version 7” and was exported to SPSS version 23 statistical software for analysis and the results were explained by percentages, tables, and frequencies. Chi-squared (test was used for comparison of categorical variables. Multivariate logistic regression analysis was conducted. The crudes odds ratio was computed (COR) and the corresponding Adjusted Odds Ratios (AOR) and 95% Confidence Intervals (CI) were used to identify factors independently associated with anemia. In all cases, Pvalue less than 0.05 is taken as statistically significant.

Operational definition

Anemia is defined according to the WHO criteria: Hgb concentration <13 g/dl for males and <12 g/dl for females. It was further classified into mild anemia (female: 11 g/dl-11.9 g/dl; male: 11 g/dl-12.9 g/dl), moderate anemia (8 g/dl-10.9 g/dl) and severe anemia (<8 g/dl).

Results

Socio-demographic characteristics of the participants

A total of 356 Type 2 diabetic patients took part in the study, with a response rate of 92.9 %. The main reasons for the nonresponse were a failure to follow through, COVID-19, and a refusal to participate. Males made up 195 (54.8%) out of the participants. The average age of the participants was 48.25 years (SD=11.401), with the dominant 128 (36%) age group was 40 years-49 years. Out of the total participants 281 (78.9%) were married and 129 (36.2%) government employers. There are 144 (40.4%) participants from the total sample having an educational level college and above and 293 (82.3%) were living in the urban area.

Lifestyle of the participants

In this study, 82 (23%) of the participants had smoked cigarettes at least once in their lives, and 41 (11.5%) of the participants were active smokers. Similarly, 191 (53.7%) of the study subjects had used alcohol at least once in their lives, and 49 (13.8%) of the participants were drinking alcohol currently. Most of the participants 18 (36.7%) were drinking two times per week. Of the total study participants, 166 (46.6%) were engaged in physical activity, and walking 134 (80.7%) is the major physical activity used by the participants. The majority of the study subjects were overweight 149 (41.9%).

Clinical conditions, complications, and comorbidities of the participants

The study showed that the duration of diabetes in most 217 (61%) of the participants was ≥5 years. All the patients were taking drugs for the treatment of their diabetes. Metformin was the most commonly used drug. Among the patients, 173 (48.6%) taking it. Besides this, 166 (46.6 %) of the patients were taking medication for the management of diseases other than diabetes, and 100 (60.2 %) of the patients were taking antihypertensive drugs. Regarding the diabetes related complications, 33 (9.3%), 69 (19.4%), 159 (44.7%), 47 (13.2%), 22 (6.2%), 105 (29.5%), and 14 (4.4%) of the patients had neuropathy, retinopathy, nephropathy, macro-vascular complication, foot sores, history of hypertension and a history of HIV/AIDS respectively. In addition, 57 (16%) of the patients have a history of blood loss. Of the study participants, 229 (64.3%) of the patients had poor glycemic control. Similarly, 105 (29.5%) had a systolic blood pressure of ≥140 mmHg, and 33(9.3%) had a diastolic blood pressure of ≥ 90mmHg. On the other hand, 147 (41.3%) of the participants had positive urine albumin, and 117 (32.9%) of the participants had higher or abnormal serum creatinine.

Magnitude of anemia and associated factors

The results of the hemoglobin test showed that 98 (27.5%) of the participants were anemic. Males (78.6%) had a higher proportion of anemia than females (22.4%). Furthermore, it was greatest in patients aged 50 years and above (64.3%) and lowest in those aged less than 50 years (35.7%). Out of anemic T2DM patients, 91 (92.9%) and 7 (7.1%) had mild and moderate anemia, respectively. To classify variables linked to anemia, bivariate and multivariate analyses were used in binary logistic regression. As a result, having nephropathy, being male, having diabetes for five years or longer, and having a high serum creatinine level were identified as associated factors for anemia in T2DM patients. Male patients were 4.32 times more likely to have anemia than females (AOR=4.32, CI: 1.09, 517.03). The occurrence of anemia was 6.2 times higher among patients who had nephropathy (AOR=6.2, CI: 1.82, 21.16). Patients with high serum creatinine (AOR=10.07, CI: 2.89, 35.2) and patients have diabetes for five years and above (AOR=4.42, CI: 1.27, 15.45) were significantly associated with anemia.

Discussion

In this Hospital based cross-sectional study, the prevalence of anemia and its associated factors among T2DM patients at Ambo Town government hospital, West Shewa Ethiopia, has been assessed. A result of the hemoglobin test showed 27.5 percent of the participants in the study were anemic. Out of anemic T2DM patients, 54 (87%) and 8 (13%) had mild and moderate anemia, respectively. Being male, diabetic nephropathy, high serum creatinine, and duration of diabetes greater than or equal to five years were identified as associated factors of anemia among T2DM patients.

Anemia is a common finding in patients with diabetes and harms the patient’s sense of wellbeing; it also impairs the ability to work and reduces the quality of life. Out of the total participants in this study, 27.5% of the diabetes patients had anemia. This finding is similar to a previous study done in Iran, which showed a 30.4% prevalence of anemia among diabetes patients. This is also consistent with a study conducted in China and Kuwait which is 22.8%, 29.7% respectively showed the prevalence of anemia among diabetes patients.

However, it was higher than other study conducted from various regions of the world, in Iran (19.6%), India (12.3%), Nigeria (15.3%), Debre-Berhan (20.1%), Kuwait (13%) and Gondar (8.6%). These differences might be due to socio-demography, feeding habits, duration of DM, and age of the study participant.

On the other hand, the magnitude of anemia in this study was lower compared to the findings of other studies conducted in Brazil (34.2%), Sub-Saharan Africa (Cameroon) (41.1%), Pakistan (63%), Harari Region (34.8%), Ghana (84.8%), Egypt (63%), And United Kingdom (59%). These differences might be due to socio-demographic, feeding habits, lifestyle, measurements, duration of DM, age of the study participants, and altitude could all contribute to the variations.

Gender was shown to be significantly associated with anemia in the current study. Male patients were 4.32 times more likely to have anemia than females. Similar findings have been reported in Harari, Gonder, and the USA. However, another analysis conducted in Pakistan and India contradicted this finding. The possible explanation could relate to the number of anemic type two diabetic female participants in this study is small and many anemic type diabetic females are in the age of menopause.

Even though there is no significant association between anemia and age among type two diabetes, this study showed the prevalence of anemia is greater in older age. This is consistent with other related studies done in Northeast Ethiopia, Kuwait Northeast, Ethiopia, and Australia. This result was anticipated since aging is related to decreased hemoglobin levels and an increase of anemia irrespective of health status. It also may be related to deficiencies of vitamins such as folate, bone marrow abnormality, and a higher number of comorbidities, which are common in the elder.

In this study, anemia is more prevalent in hypertensive patients, while no significant association between anemia and hypertension by multivariate logistic regression. The main cause could be diabetic patients, the risk of renal impairment, thus increasing the subsequent development of anemia. In addition, nutritional deficiencies especially iron deficiency and chronic inflammation can be the cause. This finding is consistent with a cross-sectional study performed in Ethiopia.

Although drug therapy which is taken for a disease other than diabetes such as, ACE inhibitors and beta are associated with anemia there is no significant association between anemias and thus drugs in this study this is inconsistent with a descriptive and analytical study done in Brazil that showed there is a significant association between body mass index and anemia. Another studies done in China and Ethiopia showed that there is a significant association between anemia and high serum creatinine level which contradicts this study.

Anemia was 6.2 times more common in patients with nephropathy, which is consistent with research from Malaysia, Iran, and Harari. This may be related to the erythropoietin hormone because erythropoietin is a hormone made by the kidney and if the kidney is not working properly, there may not be sufficient hormone produced; as a result low number of RBC precursors are produced which result in anemia. Anemia is a well-known complication of diabetes related Chronic Kidney Disease (CKD) and related to the degree of renal impairment, mainly due to impaired production of erythropoietin by peritubular fibroblast of the kidney. The finding in this study further supports this principle, as the results showed a gradual increase in the prevalence of anemia with a progressive reduction in renal function. There are many potential mechanisms, by which anemia can exist in patients with reduced renal function tests. Damage to erythropoietin producing cells through either fibrosis or chronic inflammatory activities; tubule interstitial changes; and autonomic neuropathy, which prevents anemia detection by peritubular fibroblasts of the kidney is the possible mechanism.

Our finding shows duration of DM has association with the prevalence of anemia. A positive relationship between the duration of DM and anemia with a higher chance in patients with ≥ 5 years. Compared with patients with <5 years duration of DM, the odds ratio of developing anemia in individuals with ≥5 years was 4.42 times. This finding is consistent with the previous research finding from Harari Region and Iran. The reason for this increased chance of anemia may be due to the chronic effects of hyperglycemia as a diabetes related chronic hyperglycemia can cause a chronic hypoxic milieu in the renal interstitium and disturbance of the interstitial organization of vascular architecture, atypical cell growth, and collagen proliferation in tubular cells and peritubular fibroblasts, which cause the impaired synthesis of erythropoietin by the peritubular fibroblasts. In addition, in patients with prolonged hyperglycemic conditions, the erythrocyte precursor cells in the bone marrow might be exposed to prolonged direct glucose toxicity leading to disturbances in the erythrocyte production.

The multivariate analysis of this study showed that having high serum creatinine is independently associated with greater odds for the presence of anemia. A patient with high serum creatinine was 10.07 times more likely to develop anemia and this is consistent with other related studies done in northeast Ethiopia and Ghana. Elevated creatinine level signifies impaired kidney function or kidney disease. As the kidneys become impaired for any reason, the creatinine level in the blood will rise due to poor clearance of creatinine by the kidneys. Abnormally high levels of creatinine thus warn of possible malfunction or failure of the kidneys. Reduced secretion of EPO due to renal insufficiency is the primary reason for anemia that happen due to the failing of kidney. Anemia due to kidney failing occurs when creatinine clearance is less than 50 mL/ minute; it’s observed earlier in patients with diabetes with renal insufficiency or disease. The high incidence of anemia may also be due to other risk factors related to DM. Several studies have reported factors that increase the risk of anemia, which include; damage to renal interstitium due to chronic hyperglycemia and consequent formation of advanced glycation end products by increased reactive oxygen species, and systemic inflammation as well as reduced androgen levels induced by diabetes.

Conclusion

The study showed that 27.5 % of participants were anemic. In this study anemia is more common in diabetes mellitus particularly in diabetic male’s patients and is a frequent complication of diabetic nephropathy. Out of anemic T2DM patients, 92.9% and 7.1% had mild and moderate anemia, respectively. Having nephropathy, being male, duration of diabetes for 5 years and greater, having high serum creatinine were all significantly associated with anemia.

Limitations

The study lacks control groups and did not assess the causes of anemia in diabetic patients; due to the nature of the study design, cross-sectional studies. The other limitation in this study is, morphological classification of anemia was not done due to budget problems.

Recommendation

The results suggest all Health institutions or diabetic clinics must incorporate routine screening for anemia in all T2DM patients mainly for patients with these identified risk factors to facilitate early detection and management of anemia among T2DM and consequently improve the overall care of these patients. Keeping diabetes under control and proper investigations to identify anemia in diabetic patients at an early stage can reduce the severity of the complications caused due to anemia in a diabetic population. Diabetic males and patients with nephropathy are the most vulnerable group to anemia, thus care should be taken in terms of their nutrition and supplements. Further, a longitudinal study is needed to assess the relationship over time, and to elaborate the mechanisms of anemia in patients with diabetes.

Ethical Considerations

Ethical clearance was obtained from the Addis Ababa university, college of health sciences, department of medical laboratory sciences, Department Research and Ethical Review Committee (DRERC). Permission was obtained from administrators of hospitals involved. The study aim, benefit, risk, and right for withdrawal any time from the study was explained to the study participants, and informed verbal, as well as written consent was obtained from each study participant. Physicians were informed about anemic and renal disease patients for proper management. Samples were coded and confidentiality of patient data was maintained throughout the study. Type two diabetic patients who are with mild anemic 7.1 % were got treatment.

Acknowledgments

First we acknowledge the department of medical laboratory sciences, college of health sciences Addis Ababa university, for giving us the opportunity.

We are thankful to the Ambo university referral hospital and Ambo general hospital staffs specially, lab staffs, physicians as well as Ambo town health office who contributed much for this thesis writing.

Our heartfelt gratitude also goes to study participants for giving me important information. Last, but not least my thanks also go to data collectors for their fruitful works.

Declaration of Competing Interest

We, the authors declare that there is no conflict of interest.

Consent for Publication

Not applicable.

Availability of Data and Materials

The datasets generated and/or analyzed during the current study are not publicly available due we do not have consent from all patients and ethical approval committees to publish this data] but are available from the corresponding author on reasonable request.

Funding

Not applicable.

Authors’ Contributions

JO, FU, and MH conducted the literature search, collected and analyzed the data, and wrote the manuscript. JO and FU contributed to the conception and design of the study, the interpretation of data, and critically reviewed the manuscript. JO participated in the writing, analysis and data interpretation, in addition to providing critical input to the research. FU conceptualized and designed the study, supervised the survey team and data analysis, participated in data analysis, contributed to the discussion, reviewed and edited the manuscript, and provided critical data interpretation. All authors read and accepted the final manuscript.

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