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Mechanical and Hormonal Canopy Manipulation Strategies for Improving Solanum tuberosum Andigena Tuber Yield under Limited and Unlimited Water and Nitrogen
Journal of Plant Biochemistry & Physiology

Journal of Plant Biochemistry & Physiology
Open Access

ISSN: 2329-9029

+44 20 3868 9735

Research Article - (2022)Volume 10, Issue 2

Mechanical and Hormonal Canopy Manipulation Strategies for Improving Solanum tuberosum Andigena Tuber Yield under Limited and Unlimited Water and Nitrogen

Isaac Fandika*
 
*Correspondence: Isaac Fandika, Department of Agricultural Research Services, Kasinthula Agricultural Research Station, Chikwawa, Malawi, Email:

Author info »

Abstract

Solanum tuberosum ssp. (Andigena) possesses good processing, health attributes and potential genetic diversity for breeding. However, they are handicapped by low yields due to large vegetative growth and loads of tubers per plant. This field study examined the consequences of canopy manipulation on S. andigena tuber yield, dry matter partitioning and water use efficiency (WUE) under limited and unlimited water and nitrogen (N) environment. The experiment was conducted at Massey University, Palmerston North, New Zealand from October 2010 to April 2011 comparing canopy manipulations subjected to two water and N regimes in a RCBD split-split plot design with four replicates. Solanum andigena ssp known as Tutaekuri was mechanically topped and foliar sprayed with CCC for 25-50 days from planting compared to a normal growing as a control. The water regimes were irrigation and rain-fed while applied. Topping and spray of CCC improved water use, tuber yield and WUE by increasing partitioning of dry matter to the roots and tubers. The 25-30 CCC schedule and mechanical topping adapted plants to water stress by partitioning more assimilates to the roots while 25-50 CCC schedule impeded roots development. Exclusion of N enhanced WUE while irrigation had no effect on WUE. The study suggested that excessive vegetative growth is responsible for low yields and low WUE in S. andigena and that it can be reversed by mechanical topping and CCC spray and N exclusion.

Keywords

Solanum tuberosum ; Andigena; Chlorocholine chloride; Irrigation; Nitrogen; Mechanical topping; Tuber yield; Water use efficiency; Dry matter partitioning

Introduction

The Solanum tuberosum L. subsp. andigena (Hawkes) cultivar known as Tutaekuri or Urenika in New Zealand is a distinctive heritage potato cultivar, Taewa. It is the most widely cultivated heritage potato in New Zealand. Its tubers are elongated with a dark purple skin and they have a very flourly flesh which fragments when boiled. The Solanum andigena plant also exhibits long stolons, very deep eyes, late tuberisation, large shoot biomass, many branches, many small tubers per plant and insensitivity to full irrigation compared to modern potato cultivars. Researchers described Tutaekuri as one of the heritage cultivars still exhibiting the true ancestral characteristics of the Andean potatoes. Solanum andigena also possess high antioxidant activity, high tuber dry matter content and specific gravity as observed in Tutaekuri [1,2]. However, S. andigena is handicapped by small tuber size and low yields. The high above-ground biomass suggests that S. andigena has the potential for yield improvement, through the manipulation of its shoot biomass. It was hypothesised that S. andigena species utilizes a greater proportion of assimilates for aboveground dry matter production, rather than for tubers. Subsequent reduction of the leaf canopy, either by application of growth regulatory hormones or mechanical control, would stimulate increased allocation of assimilates to tubers. This field experiment examined the consequences of mechanical topping and CCC foliar application on dry matter partitioning, tuber yield and water use efficiency in S. andegina var. Tutaekuri, under limited and unlimited water and N environments [3]. The purpose for this study was to improve the yield of Solanum tuberosum ssp. Andigena.

Materials and Methods

Treatments and experimental design

The field experiment was a RCBD split-split-plot with two water regimes as the main treatments (rain-fed and full irrigation); four canopy manipulations as sub-treatments; and two fertilizer types as sub-sub-treatments. It was replicated four times. Both fertilizer treatments received PK as a basal dressing and N was applied as a side dressing of urea. The four canopy manipulation treatments were:

• Normal growth as a control (NGC)

• Application of CCC (2-chloroethyltrimethyl-ammonium chloride (2000 ppm), twice during the tuber initiation stage at 25 and 30 days after plant emergence (DAE), coded as 25-30 CCC

• Application of CCC (2000 ppm) twice during the tuber initiation stage at 25 and 50, coded as 25-50 CCC

• Mechanical topping-It was implemented by cutting the shoots on top of the potato bush by one third of the plant

• Chlorocholine chloride was applied using a backpack sprayer at 350 liters of water per hectare.

Irrigation and fertilizer application

The irrigation treatment received 25 mm irrigation at 30 mm soil moisture deficit (SMD). Irrigation was applied by Trail boom traveller irrigator. Crop water use, for irrigated and rainfed treatments, was determined by the soil water balance approach [4]. All plots (3 m by 1.5 m each) received 30 kg P and 75 kg (at planting) of Potash Super 30% fertilizer applied. The treatment received urea (as a side dressing). Mounding (for plants at 75 cm between rows and 30 cm spacing within rows, at a depth of 10-15 cm), followed side dressing on the same day. Weeding and plant protection were carried out accordingly.

Measurements on physiological characteristics and biomass partitioning

The above-ground (leaves and stems) and below-ground biomass (roots and tubers) were sampled to determine the effect of leaf canopy manipulation on partitioning of dry matter to leaves, stems, roots or stolons and tubers. One small and one large plant sample was carefully uprooted from each plot using a spade. They were partitioned into leaves, stems, roots and tubers and then weighed and oven dried, until there was no further weight loss. The contribution of each component to total biomass on dry matter basis was determined as the ratio of each partition to total biomass (leaves, stems, roots and tubers) per plant [5,6]. The HI was calculated as the ratio of total tuber dry matter to total biomass on dry matter basis from the five samples of each plot. Leaf area (was determined using a leaf area meter), plant height (cm) and number of stems and their diameter (mm) were measured at physiological maturity. Potato psyllid was visually scored on a scale of 0-5, with 0 representing no infection and 5 being the highest infections, per experimental unit. Volumetric soil moisture was monitored using a TDR. Water use efficiency was determined as the ratio of total tuber yield to actual crop water use.

Potato tuber yield and statistical analyses

Data on tuber yield and yield components were measured at harvest. These data and those on the physiological characteristics were analyzed with the GLM procedure of the SAS differences amongst treatment means were compared by the LSD, at the 5% probability level [7].

Results

Crop water use

Tutaekuri growing season was 170 days, with a potential crop water requirement of 590 mm (Table 1). A seasonal precipitation of 368 mm contributed 63% of the total crop water requirement. Consequently, the potential crop water requirement, to be met by irrigation was 278 mm. The ratio of actual water use over potential evapotranspiration per crop stage indicated water stress in the rain-fed treatment, at the vegetative (64%), development (52%) and mid-stages (65%). Volumetric soil moisture (%) Volumetric soil moisture content (%) was significantly influenced by irrigation, days after planting (DAP) and their interaction (Figure 1). Irrigation increased the volumetric soil moisture content, but N and canopy manipulation had no much effect on soil moisture content [8]. The interaction in soil moisture, between DAP and water regimes, was a result of differences in water inputs on different days.

Crop stages (duration in days) Establishment stage Vegetative stage Development stage Mid stage Maturity stage Total days
Irrigation (mm)
Etp (mm) 21.7 170.4 134.5 208.3 54.9 589.7
Rainfall (mm) 7 93.4 70.8 52.2 0 180.5
Dp (mm) 0 0 0 0 13.3 13.3
Rain-fed
Etp (mm) 21.7 170.4 134.5 208.3 54.9 589.7
Rainfall (mm) 7 93.4 70.8 134.8 62 368
Dp (mm) 0 0 0 0 0 0

Table 1: Potential crop Evapotranspiration (ETp), irrigation (I), Deep percolation (Dp) per crop stage of S. andegina var. Tutaekuri.

Plant-Biochemistry-volumetric

Figure 1: Change in volumetric soil moisture (%) during the growing season in S. subsp. Andigena var. Tutaekuri, 2010/2011. Note: Where error bar represents ± SEM.

Vegetative plant growth characteristics

Canopy manipulation significantly affected plant height and the number of branches per plant. Mechanical topping of the canopy significantly reduced plant height, whilst application of CCC on different days reduced it intermediately, compared to the control which had the greatest plant height.

Dry matter production and partitioning

There were significant differences between canopy manipulation treatments in the partitioning of fresh and dry matter to the leaf, stem, roots, tuber and total biomass. Irrigation enhanced tuber production, total biomass production and fresh stem biomass production per plant [9].

Tuber yield and yield components at final harvest

Canopy manipulation strongly affected total tuber yield, marketable tuber yield, number of tubers per plant, and final HI. However, it did not affect mean tuber weight for total and marketable yield. The control had the lowest total and marketable tuber yield, number of tubers per plant, and final HI. Canopy management greatly improved marketable tuber yield. There was a significant interaction between irrigation and canopy manipulation on total tuber yield.

Water use efficiency and irrigation water use efficiency

Water use efficiency was highest in 25-30 CCC, which was significantly higher than the control and mechanical topping. Water use efficiency was not significantly influenced by irrigation. There was an interaction between irrigation and canopy manipulation [10].

Discussion

Plants treated with CCC managed to assimilate more dry matter to the tuber than mechanical topping, by reducing LAI, plant height and number of branches [11]. Apart from reducing excess plant height and LAI, mechanical topping concurrently increased the partitioning of dry matter to auxiliary branches and stems, thereby partially reducing its allocation to tubers, compared to the level of CCC. The current study suggests that various growing shoots in S. subsp. andigena var. Tutaekuri have powerful sinks that result in competition with the belowground sinks and tubers, when regulating the partitioning of assimilates. Spraying of CCC on leaves and the mechanical topping of growing shoots enhances the redirection of dry matter assimilates, from the excessive growing shoots to the tubers. Canopy manipulation induced S. subsp. andigena var. Tutaekuri to respond to irrigation, through increased stem size and partitioning of dry matter to roots and tubers. Irrigation and large vegetative growth increases soil moisture and relative humidity, thereby creating a desirable micro-climate environment for pests and disease incidence. Consequently, canopy manipulation minimised the N effect of decreasing dry matter partitioning to the tuber, through the rearrangement of assimilate distribution to the tubers. However, a combination of N and canopy management is an ideal strategy for S. subsp. andigena var. Tutaekuri optimum translocation of assimilates to tubers.

All canopy manipulation treatments achieved high tuber yield, by increasing the number of tubers per plant and HI. This study implies that obstruction of excessive shoot growth in S. subsp. Andigena var. Tutaekuri enhances photoassimilates partitioning into tubers, thereby boosting tuber growth. CCC promotes tuberisation by reducing the level of gibberellins, which are reported to inhibit tuber formation and which promote vegetative growth in S. andegina. On the other hand, this study indicated that higher number of tubers per plant in modified crops than control did not affect tuber yield, which was never reported in referred studies.

Conclusion

The study has examined the consequences of mechanical topping and foliar application of growth regulator and compared it with normal growth on tuber yield and WUE in S. subsp. andigena var. The results indicate that both growth regulator schedules and mechanical topping reduced excessive vegetative growth, whilst increasing the translocation of dry matter to the tubers, compared to the control but with different mechanisms. Irrigation facilitated assimilation to tubers whilst the addition of N reduced HI, due to increased and excessive vegetative growth. The results also suggest that the management of canopy increases the total tuber yield. It is recommended that farmers should be managing the canopy of S. subsp. andigena in order to optimise its crop water use and tuber productivity.

References

Author Info

Isaac Fandika*
 
Department of Agricultural Research Services, Kasinthula Agricultural Research Station, Chikwawa, Malawi
 

Citation: Fandika I (2022) Mechanical and Hormonal Canopy Manipulation Strategies for Improving Solanum tuberosum andigena Tuber Yield under Limited and Unlimited Water and Nitrogen. J Plant Biochem Physiol. 10:285.

Received: 02-Feb-2022, Manuscript No. JPBP-22-52949; Editor assigned: 07-Feb-2022, Pre QC No. JPBP-22-52949 (PQ); Reviewed: 22-Feb-2022, QC No. JPBP-22-52949; Revised: 28-Feb-2022, Manuscript No. JPBP-22-52949 (R); Published: 08-Mar-2022 , DOI: 10.35248/2469-9837.22.10.285

Copyright: © 2022 Fandika I. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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