Journal of Proteomics & Bioinformatics

Journal of Proteomics & Bioinformatics
Open Access

ISSN: 0974-276X

+44 1223 790975

Susan Williams

Department of Biological Studies, Flinders University, Adelaide, Australia

  • Short Communication   
    Role of Top-Down Proteomics for Mass Measurement using Ion Trapping Mass Spectrometer
    Author(s): Susan Williams*

    Top-down proteomics is a method of protein identification that uses an ion trapping mass spectrometer to store an isolated protein ion for mass measurement and tandem mass spectrometry analysis, or other protein purification methods such two-dimensional gel electrophoresis with MS/MS. Through the study of intact proteins, top-down proteomics may detect and quantify distinct proteoforms. The name comes from the fact that DNA sequencing is done in a similar way. Intact proteins are electrospray ionized and trapped in a Fourier transform ion cyclotron resonance (Penning trap), quadrupole ion trap (Paul trap), or Orbit rap mass spectrometer during mass spectrometry. Electron-capture dissociation or electron-transfer dissociation are used to fragment samples for tandem mass spectrometry. Prior to mass spectrometry-based proteomics, effective fractionation is necessary .. View More»
    DOI: 10.35248/0974-276X.22.15.572

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