Journal of Glycobiology
Open Access
ISSN: 2168-958X
ISSN: 2168-958X
Department of Pharmaceutics and Pharmaceutical Microbiology, Usmanu Danfodiyo University, Sokoto, Nigeria
Research Article
Partial Purification, Characterization and Investigation of the Anti-Bacterial Activity of Trilepisium madagascariense Seed Lectin
Author(s): Ilemobayo Victor Fasogbon*, Micheal Nosano Yakubu, Adam Mustapha, Chidiebere Obi, Nuhu Tanko, Danjuma Ali Zenoh, Mari Batta Mshelia, Aliyu Salihu, Ismaila Alhaji Umar and Muhammad Sani Aliyu
Published data on the properties and possible applications of the Lectin(s) from Trilepisium madagascariense seed is still limited. This study aimed to partially characterize and investigate the seed extract of T. madagascariense for antibacterial activity against Bacillus cereus, Staphylococcus aureus, Pseudomonas aeruginosa and Escherichia coli, using agar well diffusion method. The extract was partially purified by Sephadex G-75 filtration and characterized, to obtain a partially purified Trilepisium madagascariense Seed Lectin (ppTMSL).The ppTMSL was found to agglutinate all human erythrocytes (blood groups A, B, and O), showing no blood group specificity; although there was statistical increase (P<0.05) in its activity on blood group O erythrocytes while comparing the natural logarithm of the mean of specific activi.. View More»
DOI:
10.35841/2168-958X.23.12.267