Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
ISSN: 2161-0495
Laboratory of Enzyme and Protein Technology, Hue University, Hue City, Thua Thien Hue Province, Vietnam
Research Article
Cloning and Expression of PirA Gene of Vibrio parahaemolyticus Strain K5 Causing Acute Hepatopancreatic Necrosis Disease in Whiteleg Shrimp in E. coli Host Cell
Author(s): Nguyen van Khanh, Tran Quoc Dung, Tran Quang Khanh van and Nguyen Quang Linh*
Acute Hepatopancreatic Necrosis Disease (AHPND) is a bacterial disease of white leg shrimp, which has a high mortality rate (100%) and economic losses. Our objective was to identify the genes which caused cell and organ toxic damage and applied bioproducts to prevent it. Litopenaeus vannamei shrimp were collected from an infected pond in Central Vietnam and analyzed at the Institute of Biotechnology, Hue University. The PirA gene of Vibrio parahaemolyticus strain K5 was isolated and analyzed for nucleotide sequence, paired with the expression vector pQE30. The expression vector was transformed into E. coli strain M15, the PirA recombinant protein was expressed in the form of 6xHis-PirA fusion protein of about 15 kDa. PirA recombinant protein was purified and determined the PirAvp binding ratio, cloning, and se.. View More»
DOI:
10.35248/2161-0495.22.12.522