Journal of Cancer Research and Immuno-Oncology
Open Access
ISSN: 2684-1266
ISSN: 2684-1266
Department of Medical Laboratory Technology, Bapubhai Desaibhai Patel Institute of Paramedical Sciences,Changa-388421, Gujarat, India
Research Article
A Systematic Biology Advances on sgRNA Design to Develop an Immunogenic Drug against Cancer Using CRISPR
Author(s): Disha Patel and Khushal Patel*
In the field of genome editing, CRISPR/Cas9 is more suitable for clinical therapeutic applications, especially in the treatment of cancer. Here we wanted to design sgRNA that targets specific genes of helper T cells and cytotoxic T cells to alter them so that they can attack tumor cells. In other words, activating the immune system to treat cancer through the development of an immunogenic living drug. Among the main methods, the sgRNA of the target genes (PDCD-1, CTLA-4 and NYESO-1) was designed using the bioinformatics tool Benchling. The designed sgRNA was then cloned in silico into a lentivirus-based vector using the SnapGene gene designer. Isolation of an immunogenic drug can be done by transferring designed clones in vitro into the host and isolating pseudo-HIV viruses from the host, which consists of designed sgRNAs along with the CRI.. View More»
DOI:
10.35248/2684-1266.25.11.238