Department of Microbiology, Obafemi Awolowo University, Osun, Nigeria
Pseudomonas aeruginosa Associated With The Hospital Fomites In Ile-Ife, Osun State Nigeria.
Author(s): Joseph Omololu-Aso*, K. Aliu, Oluwaseun O. Omololu-Aso and Oluwagbenro Adesunloro
The aim of this study was to isolate, characterize and identify Pseudomonas aeruginosa from hospital fomites. A total of fifty samples were collected from door knobs/handles (10), stethoscopes (10), hospital rails (10), patient beddings (10) and sphygmomanometer (10) using sterile swab sticks at the Obafemi Awolowo University Teaching Hospitals Complex (OAUTHC), Ile-ife, Osun-state. Samples were cultured on cetrimide agar and incubated at 37°C for 24 hrs. Pseudomonas aeruginosa were isolated and identified based on growth on cetrimide agar, gram’s reaction, and positive results for catalase, oxidase, citrate, Triple Sugar Iron (TSI), mannitol fermentation test and negative results for MRVP. Eight Pseudomonas aeruginosa isolates were recovered out of sixteen presumptive Pseudomonads based on positive results on cetrimide agar. The.. View More»