Journal of Fertilization: In Vitro - IVF-Worldwide, Reproductive Medicine, Genetics & Stem Cell Biol
Open Access

Department of Biological Sciences, Ale Taha University, Tehran, Iran

Publications

• Research Article   
  Functional Study of the Effect of miR-647 on the Prognosis of Polycystic Ovarian Syndrome (PCOS)
  Author(s): Hanieh Motahari Rad*, Fatemeh Akbarian, Zohreh Hashemian, Seyed Javad Mowla and Mojtaba Rezazadeh Valojerdi
  
  Purpose: Polycystic Ovarian Syndrome (PCOS) is a metabolic syndrome that is regulated by several genes and miRs. In a previous study, we showed that miR-647 was significantly downregulated in PCOS patients compared with normal controls. Furthermore, miR-647 exhibited a negative correlation with CYP11A1 in both follicular fluid and granulosa cells. The purpose of this study was to gain a preliminary understanding of the role of miR-647 in granulosa cells and its impact on the prognosis of PCOS patients. Methods: A miR-647 Precursor (premiR) and a Sponge (miR-sp) were designed for the overexpression and knockdown of miR-647. Wild-type and mutant 3'-UTRs of CYP11A1 were also prepared as specific binding sites for miR-647. On the basis of bioinformatics studies, the low level of miR-647 in HEK-293T cells makes it suitable for testing the eff.. View More»
  

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