Journal of Clinical Toxicology

Journal of Clinical Toxicology
Open Access

ISSN: 2161-0495

+44 7868 792050

Didier Desmaële

Laboratoire Chrono Environnement UMR CNRS 6249, Université de Bourgogne Franche- Comté, 16 route de Gray, 25030 Besançon Cedex, France

  • Research Article   
    Optimizing Structure and Activity of the Squalenoyl-siRNA Nanoparticles
    Author(s): Suzan Boutary*, Guy Khalaf, Marie Caillaud, Didier Desmaële, Semen Yesylevskyy, Christophe Ramseyer and Liliane Massaad-Massade

    siRNA is a promising tool for targeted therapy. Previously we conjugated the siRNA to Squalene (SQ) by copper- free click chemistry, which has been proven to successfully deliver siRNA to tumors with fusion oncogenes and Schwann cells. To go further for future human application, this study aims to optimize the synthesis and the nanoprecipitation of siRNA-SQ conjugates while conserving their physicochemical characteristics and efficiency. We succeeded in increasing the yield of the siRNA-SQ conjugation reaction making it scalable up to 50-times. The size, the polydispersity index and the ζ-potential of the nanoparticles obtained are strongly dependent on the solvent used. Moreover, the molecular dynamics simulations reveal the physical mechanisms of nanoparticle formation at the early stage of their growth. However, these parameters do not influence the activity of the nanopart.. View More»
    DOI: 10.35248/2161-0495.23.13.530

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