Journal of Clinical Toxicology
Open Access
ISSN: 2161-0495
ISSN: 2161-0495
Department of Biochemistry, Jinnah Sindh Medical University, Karachi, Pakistan
Research Article
Prognosis of Hepatocellular Carcinoma with the Detection of Glypican 3 and its Inhibition by Triptolide using Docking Studies with Autodock Vina
Author(s): Iffat Ara*, Burhan Khatri, Qurat Ul Ain Chaudhary, Shakeel Ahmed and Khadija Makkiah
Intake of fluoridated water can be one of the most common neglected factors affecting the livers of individuals. Hepatocellular Carcinoma (HCC) is the most common malignant tumor responsible for a large number of deaths globally. Surveillance requires the early detection of HCC among patients at the first stage of cancer. The study aimed at the prognosis of HCC for which the Glypican-3 (GPC3) biomarker was used. Fluoride content in drinking water and serum of populations from district Narowal (sample) and DHA Lahore (control) was measured by the ion-selective electrode. The levels of expression of GPC3 in both samples and controls were determined by ELISA. The binding affinity of triptolide to glypican 3 using AutoDock Vina was determined by hydrogen bonds, binding energy, and clusters of interacting amino acid residues using discovery studio. Results analyzed by student’s t-tes.. View More»
DOI:
10.35248/2161-0495.23.13.534