Biochemistry & Pharmacology: Open Access
Open Access

Vidya Patni

Publications

• Research Article
  Identification and Qualitative Analysis of β-Sitosterol and Some Phytoestrogens in In Vivo and In-Vitro Samples of Lepidium sativum: A Semi-Arid Bone Healing Plant
  Author(s): Raghunandan S Nathawat, Preeti Mishra and Vidya Patni Raghunandan S Nathawat, Preeti Mishra and Vidya Patni
  
  Dry powder of different plant parts of Lepidium sativum (Garden Cress) are known to be used for treatment of fracture (bone healing) from ancient times. Seeds as in vivo plant part and callus as in vitro plant parts were chromatographically tested to identify and estimate β-sitosterol and various phytoestrogens. Plant-derived sterols and estrogens in tissues and oilseeds of Lepidium sativum were isolated by solvent extraction method. β-sitosterol, diadzein and formononetin were estimated qualitatively and quantitatively through TLC and HPTLC techniques. In vitro grown callus was compared biochemically with in vivo plant parts. CAMAG HPTLC was used and the developed plates were photo-documented using UV and white light. The amount of β-sitosterol was estimated to be about 0.20% w/w for seed powder and 0.024% w/w for callus powder. Daidzein and formononetin isolated from .. View More»
  DOI: 10.4172/2167-0501.1000191

  Abstract PDF

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