Anatomy & Physiology: Current Research
Open Access
ISSN: 2161-0940
+44 1300 500008
ISSN: 2161-0940
+44 1300 500008
Van Eck M
Netherlands
Research Article
Comparison of Hormones, Lipoproteins and Substrates in Blood Plasma in a C57bl6 Mouse Strain after Starvation and a High Fat Diet: A Metabolomics Approach
Author(s): Van Ginneken V, Ham L, Evert de Vries, Elwin Verheij, Jan van der Greef and Van Eck MVan Ginneken V, Ham L, Evert de Vries, Elwin Verheij, Jan van der Greef and Van Eck M
Male C57bl6 mice (age 8-12 weeks) were fed a high-fat diet, containing 0.25% cholesterol (Ch) and 24% energy from bovine lard for a period of approximately 40 days or were exposed to 24 hours of starvation. Exposure to 24 hours of starvation, resulted in a significant drop in plasma glucose (1.8-fold), Phospholipids (PL) (1.2-fold), Triacylglycerols (TG) (1.4-fold), Total Cholesterol (TCh) (1.4-fold), Free cholesterol (FCh) (1.4-fold) and a significant increase in Ketone bodies (9.6-fold). After 24 hours of starvation plasma Very Low Density Lipoproteins- (VLDL), Low Density Lipoproteins-(LDL) and High Density Lipoproteins-(HDL) TCh and-Phospholipids (PL) were unaffected, while VLDL TG was significantly decreased and LDL and HDL TG significantly increased. Plasma Free Fatty Acids (FFA) and Insulin were unaffected. In the high-fat diet group plasma VLDL-, LDL- and HDL-TCh and -PL were .. View More»
DOI:
10.4172/2161-0940.1000233