Endocrinology & Metabolic Syndrome
Open Access
ISSN: 2161-1017
+44 1478 350008
ISSN: 2161-1017
+44 1478 350008
Tsuyoshi Chiba
National Institutes of Biomedical Innovation,
Health and Nutrition, 1-23-1 Toyama, Shinjuku-ku, Tokyo 162-8636
Japan
Research Article
The Major Cytochrome P450 Subtype Activities in Diet-Induced Non- Alcoholic Steatohepatitis Mouse Model
Author(s): Sachina Suzuki, Yoko Sato, Keizo Umegaki and Tsuyoshi ChibaSachina Suzuki, Yoko Sato, Keizo Umegaki and Tsuyoshi Chiba
Objective: Non-Alcoholic Steato Hepatitis (NASH) is now recognized as a global liver disease. Medication combined with diet and exercise regimen is used to treat NASH, because NASH is associated with metabolic syndrome, such as insulin resistance or dyslipidemia. However, it currently remains unclear whether NASH affects the metabolic power and activity in the liver especially for hypo-cholesterolemic drugs. To address this issue, we measured major Cytochrome P450 (CYP) subtype activities in mice with diet-induced NASH.
Methods: C57BL/6J mice (male, 8 weeks of age) were fed a High-Fat and High-Cholesterol (HFHC) diet or normal chow diet (control) ad libitum for 12 weeks. Plasma lipid levels and hepatic lipid contents were then measured. The mRNA expression levels of the CYP subtypes and inflammatory cytokines in the liver were assessed using qu.. View More»
DOI:
10.4172/2161-1017.1000190