Gynecology & Obstetrics
Open Access
ISSN: 2161-0932
ISSN: 2161-0932
Sirisha P
Institute of Genetics and Hospital for Genetic Diseases,
Begumpet, Hyderabad - 500016
India
Research Article
Screening of BRCA 1 - 185delAG mutation in Ovarian Cancer patients in a Tertiary care centre from Telangana
Author(s): Sirisha P, Vottery R, Amidyala L, Nallari P, Jyothy A and Venkateshwari ASirisha P, Vottery R, Amidyala L, Nallari P, Jyothy A and Venkateshwari A
Aim: Ovarian cancer (OC) occurs due to genetic alterations and mutations in BRCA gene. The aim of the study is to assess the frequency of genetic alterations that persist in Ashkenazi founder mutation BRCA1, 185delAG in patients with ovarian cancer from South Indian origin.
Materials and Methods: A total of 100 ovarian cancer patients and an equal number of control subjects were included in the present study. Screening of 185delAG mutation BRCA1 gene was carried out by ARMS PCR followed by agarose gel electrophoresis. Statistical analysis was applied to test for the significance of the results obtained.
Results: The genotype distribution of WW, WM, MM showed a significant difference between the two subjects, 95%, 4% and 1% in controls and 52%, 36% and 12% in cases respectively. An increased frequency of homozygotic.. View More»
DOI:
10.4172/2161-0932.1000444