Gynecology & Obstetrics
Open Access
ISSN: 2161-0932
ISSN: 2161-0932
Rosenbaum ST
Holbaek Hospital, 60 Smedelundsgade, 4300 Holbaek
Denmark
Research Article
Relaxant Effect of Cyppa In Vitro and Expression Analysis of SK3 Splice Variants in the Myometrium of Pregnant and Non-Pregnant Women
Author(s): Rosenbaum ST, Svalo J, Larsen T, Joergensen J and Bouchelouche PRosenbaum ST, Svalo J, Larsen T, Joergensen J and Bouchelouche P
Background and objective: We have recently shown an overall down-regulation of SK3 channels in pregnant human myometrium compared to non-pregnant myometrium. The aim of this study was to investigate whether SK3 splice variants are involved in the down-regulation of SK3 channels. We also tested whether SK channels are implicated in myometrial contractility using CyPPA, a novel and selective SK2 and SK3 channel activator.
Study design: We evaluated the expression of SK3 splice variants at mRNA level by qRT-PCR in myometrium from pregnant (n=11) and non-pregnant (n=11) women. Isometric tension recordings were performed to assess human myometrial contractility on biopsies obtained at elective caesarean section at term (n=6) and from hysterectomy (n=6). We investigated the effects of CyPPA (0.1-60 μM) on spontaneous contractions.
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DOI:
10.4172/2161-0932.1000171