Biochemistry & Pharmacology: Open Access
Open Access
ISSN: 2167-0501
+44-20-4587-4809
ISSN: 2167-0501
+44-20-4587-4809
Pierre Mugabo
Private Bag X17, 7535. Bellville
South Africa
Research Article
Determination of Kanamycin Plasma Levels Using LC-MS and Its Pharmacokinetics in Patients
Author(s): Pierre Mugabo, Mercy I Abaniwonda, Danie Theron, Leonie Van Zyl, Shafick M Hassan, Marietjie Stander, Helen McIlleron and Richard MadsenPierre Mugabo, Mercy I Abaniwonda, Danie Theron, Leonie Van Zyl, Shafick M Hassan, Marietjie Stander, Helen McIlleron and Richard Madsen
Purpose: The objectives of the study were: (1) to determine kanamycin plasma concentrations using liquid chromatography coupled with mass spectrometry (LC-MS), (2) to investigate kanamycin pharmacokinetics (PK) in patients with multi-drug resistant tuberculosis (MDR-TB), (3) to find out whether HIV infection, kidney dysfunction and antiretroviral drugs influence kanamycin PK.
Methods: The study was designed as a non-randomized study involving male and female HIV- positive and HIV negative patients admitted for MDR-TB treatment. Blood samples were collected before (baseline) and ½, 1, 2, 4, 8 and 24 hours after intramuscular injection of kanamycin. LC-MS was used to quantify kanamycin plasma concentrations.
Results: Thirty one patients including 13 HIV (+) participated in the study. The lower limit of detecti.. View More»
DOI:
10.4172/2167-0501.1000160