PMC/PubMed Indexed Articles
Indexed In
- Open J Gate
- Genamics JournalSeek
- JournalTOCs
- Ulrich's Periodicals Directory
- RefSeek
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- Publons
- Geneva Foundation for Medical Education and Research
- Euro Pub
- Google Scholar
Useful Links
Share This Page
Journal Flyer
Open Access Journals
- Agri and Aquaculture
- Biochemistry
- Bioinformatics & Systems Biology
- Business & Management
- Chemistry
- Clinical Sciences
- Engineering
- Food & Nutrition
- General Science
- Genetics & Molecular Biology
- Immunology & Microbiology
- Medical Sciences
- Neuroscience & Psychology
- Nursing & Health Care
- Pharmaceutical Sciences
Marius Dybwad
Marius Dybwad
Norway
Publications
-
Review Article
Mature Bone-Producing Osteoblasts Alter Gene Expression of Metastasis Related Genes in Prostate Cancer Cells
Author(s): Haakon Skogseth, Marius Dybwad, Arnar Flatberg and Jostein HalgunsetHaakon Skogseth, Marius Dybwad, Arnar Flatberg and Jostein Halgunset
Background: Prostate cancers often metastasize to bone. It is hypothesized that interactions between osteoblasts and cancer cells lead to establishment of prostate cancer skeletal metastasis. Methods: As a result of co-culture between undifferentiated and differentiated osteoblasts and PC-3 and DU-145 prostate cancer cells, gene expressional changes were determined with microarray technology, both Affymetrix® and Illumina® protocols. Characterization of osteoblast differentiation was performed using alizarin red staining of calcium, saffron staining of collagen fibers and quantitative real-time PCR of differentiation markers (RUNX2, Osteocalcin and Alkaline phosphatase). Results: The Affymetrix® protocol demonstrated that co-culturing of PC-3 cells with differentiated osteoblasts resu.. View More»
DOI: 10.4172/2157-2518.1000111