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Jostein Halgunset
Jostein Halgunset
Norway
Publications
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Review Article
Mature Bone-Producing Osteoblasts Alter Gene Expression of Metastasis Related Genes in Prostate Cancer Cells
Author(s): Haakon Skogseth, Marius Dybwad, Arnar Flatberg and Jostein HalgunsetHaakon Skogseth, Marius Dybwad, Arnar Flatberg and Jostein Halgunset
Background: Prostate cancers often metastasize to bone. It is hypothesized that interactions between osteoblasts and cancer cells lead to establishment of prostate cancer skeletal metastasis. Methods: As a result of co-culture between undifferentiated and differentiated osteoblasts and PC-3 and DU-145 prostate cancer cells, gene expressional changes were determined with microarray technology, both Affymetrix® and Illumina® protocols. Characterization of osteoblast differentiation was performed using alizarin red staining of calcium, saffron staining of collagen fibers and quantitative real-time PCR of differentiation markers (RUNX2, Osteocalcin and Alkaline phosphatase). Results: The Affymetrix® protocol demonstrated that co-culturing of PC-3 cells with differentiated osteoblasts resu.. View More»
DOI: 10.4172/2157-2518.1000111