Biochemistry & Pharmacology: Open Access
Open Access
ISSN: 2167-0501
+44-20-4587-4809
ISSN: 2167-0501
+44-20-4587-4809
Hitoshi Mineo
Eniwa, Hokkaido 061-1449
Japan
Research Article
Carboxylic Acids with Certain Molecular Structures Strengthen the Cell Membrane against Osmotic Pressure in Sheep Erythrocytes In Vitro
Author(s): Hitoshi Mineo, Mikako Noji, Yukino Watanabe, Yukina Yoshikawa, Yuuka Ono and Naomi IwayamaHitoshi Mineo, Mikako Noji, Yukino Watanabe, Yukina Yoshikawa, Yuuka Ono and Naomi Iwayama
In sheep red blood cells (RBCs), considering osmotic fragility (OF) as an indicator, the reaction of monocarboxylic and dicarboxylic acids on membrane resistance to osmotic pressure were evaluated. Sheep RBCs were exposed to carboxylic acids at 0-100 mM in a buffer solution for 1 h and the 50% hemolysis was then determined by soaking in 0.1-0.8% NaCl solution. Although formic acid declined and n-caprylic acid increased OF, most of the monocarboxylic acids with straight hydrocarbon chains did not change OF in sheep RBCs. Whereas, all the tested dicarboxylic acids with straight hydrocarbon chains decreased OF with the degree of the decrease in OF dependent on the compound. Some monocarboxylic acids with branched or cyclic (including a benzene ring) hydrocarbon chains decreased OF with the degree of the decrease dependent on the number of carbons and form of branching in the hydrocarbon .. View More»
DOI:
10.4172/2167-0501.1000252