Endocrinology & Metabolic Syndrome
Open Access
ISSN: 2161-1017
+44 1478 350008
ISSN: 2161-1017
+44 1478 350008
Alvin M. Kaye
Israel
Review Article
Age-Dependent Responsiveness of Human Female Cultured Bone Cells to Estrogenic Compounds
Author(s): Dalia Somjen, Sara Katzburg, Alvin M. Kaye, Fortune Kohen and Gary H. PosnerDalia Somjen, Sara Katzburg, Alvin M. Kaye, Fortune Kohen and Gary H. Posner
Human cultured female osteoblasts (Obs) respond age-dependently to estradiol-17β (E2) and to phytoestrogens by increased DNA synthesis (DNA) and creatine kinase specific activity (CK). ERα mRNA expression is higher than ERβ mRNA in Obs. Pre-menopausal Ob (prOb) reveals higher expression of ERα than post-menopausal Ob (poOb), but similar intracellular and membranal E2 bindings. ERα mRNA is stimulated in prOb and inhibited in poOb by different estrogens. ERβ mRNA in prOb is stimulated by 2,3-bis (4-hydroxyphenyl)-propionitrile (DPN; ERβ specific agonist) and 4,4ʹ,4ʺ-[4-Propyl-(1H)-pyrazol-1,3,5-triyl] tris-phenol (PPT; ERα specific agonist) and raloxifene (Ral) and inhibited by genistein (G) and daidzein (D) while in poOb only E2 and DPN inhibited it. All phytoestrogenic carboxy- derivatives stimulated ERα mRNA in both Ob, while the.. View More»
DOI:
10.4172/2161-1017.S1-001