This article refines tweaks and completes the mathematical model that allows accounting qualitatively and quantitatively for the Crabtree effect in eukaryotic cells cultured in the chemostat. To the respirofermentative phenomena, this work adds the representation of the often-observed maintenance phenomena. This extended two-way model accounts for the theoretical aspect of maintenance but also allows us to calculate the associated coefficient. We obtained, for Saccharomyces cerevisiae, a value of mGLU=0.094 h-1 very close with those of the literature. An unexpected relationship between ethanol produced by the yeast and its intracellular pyruvate concentration was highlighted, as well as the plausible independence of the yield coefficient from the maintenance coefficient, a relevant observation for optimization in biotechnological production processes.
Published Date: 2019-11-14; Received Date: 2019-10-27