Journal of Genetic Syndromes & Gene Therapy
Open Access

Abstract

The Analysis of Hormone Replacement Therapy Influence on Il-6 Expression and Mandible T-Score of Postmenopausal Women

Mansur Rahnama, Izabela Jastrzebska-Jamrogiewicz, Rafal Jamrogiewicz and Grzegorz Trybek

Objective: Aim of the study was to investigate the effect of Hormone Replacement Therapy (HRT) on IL-6 gene expression and salivary concentration, bone mineral density (BMD) and T-score (the number of standard deviations below the average for a young adult at peak BMD) of mandible in menopausal women. Materials and Methods: Study was carried out on a group of 60 women during menopause - 30 untreated (control group) and 30 treated with HRT (test group). 30 patients has undergone natural menopause and other half of women were after ovariectomy. Examination of IL-6 gene expression was conducted on peripheral blood lymphocytes (PBL) and buccal epithelial lining (BEL). Saliva samples were collected and densitometry was conducted on the mandible. To evaluate the results of densitometric examination T-score index was calculated. Results: T-score index of the control groups reached values below (-2). T-score results for test group were higher than those in control group. HRT results in a slight decrease of the IL-6 level in saliva of the test group,compared with control group, but the differences between groups were not statistically significant. Only in groups after natural menopause treated with HRT significant increase of IL-6 gene expression was stated when compared to control groups. Conclusions: HRT has a significant osteoprotective effect on the mandible. This local beneficial effect of HRT may be exerted, inter alia, by local decrease in salivary concentration of proinflammatory cytokine IL-6, but further research is required to clearly confirm this thesis. Results of IL-6 gene expression do not allow stating any clear thesis. It needs to be clarified if HRT has the same effect on cytokine gene expression as hormones produced by ovaries.