Tahira Mughal, Ismat Naeem, Sadia Qureshi , Ammara Abass
Heliotropium sterigosum is an annual herb found in south Punjab of Pakistan. Extract of whole plant in methanol was tested for anti bacterial activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus pneumoniae, Bacillus subtilus, and Sarcina lutae. Minimum Inhibitory Concentration of methanolic extract was determined and found to be bactericidal in concentration of 1000 μg/ml against streptococcus pneumoniae, Escherichia coli, and Streptococcus pneumoniae. Synergistic antibacterial activity of methanolic extracts was tested with respective solvent extracts of seeds of Withania coagulans, whole plant of Hypericum perforatum and Pinus roxburgii (bark). Staphylococcus aureus was synergistically inhibited by methanolic extract of Heliotropium sterigosum and Pinus roxburgii (bark) (1μg/ml) and Withania coagulans (1μg/ml). Methanolic extract of Heliotropium sterigosum inhibits the growth of Sarcina lutae in combination with Pinus roxburgii (bark) methanolic extract (0.5μg/ml). Highest synergistic activity was observed by Heliotropium sterigosum and Withania coagulans methanolic extracts against Pseudomonas aeruginosa (0.5μg/ml) and Bacillus subtilus (1μg/ml).