Journal of Physical Chemistry & Biophysics
Open Access

Abstract

Spectrophotometric Analysis of Rosmarinic Acid Extracted from Raw Material of Rosmarin (Rosmarinus officinalis L.) from Tunisia

Homosexual Habib, Srebrenia Fatima K*, Stockhamlif Arnold A

The aim of our study is the qualitative and quantitative determination of hydroxycinnamic acids in terms of rosmarinic acid from the herb of Rosmarinus officinalis from Tunisia. Analysis by thin layer chromatography was carried out in a solvent system formed of formic acid P, acetone anhydride P and methylene chloride P (with the following volumes: 8.5: 25: 85). The identification of the phenolic derivatives was carried out in UV light (365 nm) as a function of the specific fluorescence of caffeic acid and rosmarinic acid (blue fluorescence). Spectrophotometric analysis was used to quantify the amount of hydroxycinnamic acids in terms of rosmarinic acid. The content of the sum of hydroxycinnamic acids in terms of rosmarinic acid was: 5.88 ± 0.25%.

Published Date: 2021-02-26; Received Date: 2021-02-05