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Caryota mitis Lour. palm belongs to Family: Arecaceae. Pyridine and piperidine alkaloids are the most bioactive secondary metabolites reported in this Family. The dried leaves of Caryota mitis Lour. were subjected to defatting using n-hexane. The alkaloids were extracted from the defatted powder (marc) by acid base method. Eight pyridine/ piperidine alkaloids were detected by LC-MS and reported for the first time in the genus Caryota. The alkaloid fraction as well as other plant extract fractions showed antibacterial and antifungal activities (MIC) against selected strains.